Kwamina Nyame

This study develops LC/MS methods for accurate BMP profiling, using CLN5 knockout cells to distinguish BMP from PG and introduce BMPII and BMPES metrics.

The paper creates a cell-type-specific lysosome protein atlas in the brain, identifies SLC45A1 as a neuron-specific lysosomal protein, and shows its role in lysosomal function and neurodegeneration.

The paper identifies TFEB regulation of BMP lipid catabolism via Pla2g15, showing increased BMP lipids in Pla2g15 knockout mice and cold intolerance upon TFEB knockdown.

This study identifies PLA2G15 as a lysosomal BMP hydrolase and shows its targeting improves lysosomal disease symptoms in NPC1 models.

The paper develops a tagless LysoIP method to isolate lysosomes from clinical samples and human cell lines, revealing glycerophosphodiesters as potential biomarkers for lysosomal storage disorders.