John J. Wright

The paper defines the proton-pumping pathways in respiratory complex I from two bacteria, revealing structural determinants for redox-coupled proton uptake and explaining the stoichiometry adaptation for energy conservation.

The paper presents cryo-EM structures of bovine respiratory complex I under varying protonation conditions, revealing conformational changes at histidine junctions that coordinate proton translocation.

This study defines the structural basis of a regulatory switch in mammalian complex I by combining cryoEM structures with biochemical analysis, showing how conformational changes mediate deactivation during ischaemia.