Irina Kufareva

This study shows that knocking out PKA regulatory subunits RI and RII leads to distinct metabolic and phosphorylation changes, with RI KO increasing Tau phosphorylation linked to Alzheimer's disease.

This study compares the conformational dynamics of ACKR3 and CXCR4 using MD simulations and BRET assays, revealing that ACKR3's dynamic behavior leads to activation-proneness and G protein-incompetence.

This paper introduces GPCR-CoINPocket v2, a method to detect pharmacological similarities in GPCRs using sequence and ligand interaction data, revealing new relationships and aiding drug discovery.

The paper maps the chemokine-stimulated signal transduction network in monocytic cells and shows that chemotaxis emerges from a divergent signaling network that can be inhibited to block cell migration.

This paper identifies a host-encoded toggle switch involving NOD2 and GIV that controls cAMP levels in macrophages, converting tolerant cells into microbicidal machines to restore microbial clearance and gut barrier integrity.

This study identifies the molecular mechanisms underlying signaling bias in PAR1, showing distinct roles of GRK5 and β-arrestin-2 in thrombin- and APC-induced responses.

This paper identifies a new class of thiophen-2-yl pyrimidines (TPPs) with improved solubility and potency against Schistosoma species, suggesting novel targets beyond tubulin.

This study identifies N-terminal determinants of CCL27 and CCL28 receptor activation and selectivity by analyzing mutations and using AlphaFold models.

This study identifies a non-canonical activation mechanism of CCR9 by CCL25 through structural modeling and mutational analysis, revealing the 30s loop as the key activation region.

This paper assesses GPCR-ligand complex predictions in the era of AlphaFold, showing AI models can match or exceed low-resolution experimental structures but still need high-resolution data and hybrid methods.

This study uses APEX2 proximity labeling and mass spectrometry to uncover ligand-dependent CCR5 trafficking mechanisms, revealing distinct internalization and degradation pathways.

This study develops and validates BRET-based assays to detect constitutive and ligand-dependent Gi-directed activity in GiPCRs, revealing novel regulatory mechanisms of Smoothened.

This study identifies molecular determinants of CCR2 and CCR5 antagonist interactions using docking and AI screening, revealing structural principles and selectivity mechanisms.