Steven W. Hardwick

This paper presents a cell-free method to rapidly produce and determine cryo-EM structures of GPCR-nanodisc complexes, achieving structural-grade results in four days.

The paper identifies two protein contaminants from affinity-purified samples using cryo-EM and mass spectrometry, emphasizing the importance of resin specificity in purification.

This study identifies how DNA polymerase Lambda interacts with the NHEJ complex via Ku70/80, revealing the molecular basis for its recruitment and activity in DNA repair.

This paper identifies NACHO as an ER-resident protein that facilitates the assembly of pentameric ligand-gated ion channels by interacting with subunit interfaces.

This study reports cryo-EM structures of human mitochondrial tRNA maturation complexes, revealing the molecular mechanisms of tRNA processing steps and their sequential order.

The paper reports the cryoEM structures of the RapZ:GlmZ complex and its interaction with RNase E, revealing mechanisms of RNA regulation in bacterial cell envelope biogenesis.