Robert Sebra

The paper profiles human myeloid cells across aging and Alzheimer's disease, identifies AD-associated changes, and links GPNMB to neuroprotection via TREM2.

The paper assesses low-biomass microbiome detection in tissues using long-read sequencing, finding that genuine microbiome signals are limited to tissues with natural microbial exposure.

This study identifies dysregulated TGFβ-ERK signaling as a driver of aberrant extracellular matrix production in Noonan syndrome-associated pulmonary valve stenosis using iPSC models and patient tissue.

The paper introduces HIV SMRTcap, a method for characterizing HIV-1 reservoirs by identifying integration sites and proviral integrity using long-read sequencing, and validates its performance across subtypes and tissue samples.

The paper develops an integrative sequencing strategy to catalog NRXN1 isoforms across human brain tissues and hiPSC organoids, revealing cell-type-specific splicing patterns and therapeutic potential of ASO targeting.

The paper identifies structural variants and coding polymorphisms in the human IGHC locus, revealing population-specific diversity and potential functional impacts.

This study compares gene expression in living and postmortem human brain tissue, finding significant differences that challenge the assumption that postmortem data accurately represents living brain gene expression.

This study identifies a shared infiltrative signature in glioblastoma at the resection margin using spatial multi-omics data and validates EGFR as a key marker.

This study shows that fumarate, regulated by host and pathogen, limits S. aureus growth by blocking metabolic pathways, while FumC enables adaptation through fumarate degradation and utilization.

This study identifies TNFR1-mediated senescence and limited TNFR2 signaling as barriers to intervertebral disc cell repair in back pain, suggesting immunomodulation as a therapeutic strategy.

The paper presents a long-read RNA-seq atlas of microglia isoforms, identifying novel isoforms and their associations with genetic risk loci in Alzheimer's and Parkinson's disease.

This study identifies an oncofetal cell state that contributes to resistance in colorectal cancer and shows that targeting both this state and LGR5+ CSCs is needed for effective treatment.

This study uses deep learning and reference atlases to analyze tumor cell populations in mouse bladder cancer, identifying lineage-specific subpopulations and their therapeutic vulnerabilities.

This study uses single-cell RNA sequencing to identify cycling alpha cells as key beta cell progenitors in response to DYRK1A inhibitor treatment in human pancreatic islets.

The paper identifies cardiotoxic drug signatures using bulk transcriptomics and integrates them with genomic and single-cell data to predict toxicity mechanisms.

This paper uses single-cell RNA-sequencing to analyze the tumor microenvironment in pleural mesothelioma, identifying gene programs, fetal-like endothelial cells, and a new therapeutic target.

The paper compares gene expression differences between living and postmortem human brain samples using snRNA-seq and develops a model to account for these differences in analysis.

This study investigates how diet and stress interact with sex to affect behavior, metabolism, and the ventromedial hypothalamus in mice, revealing sex-specific effects on fear memory, glucose levels, and metabolic parameters.

The paper identifies SCI-induced changes in axonal RNA, including circular RNAs, and shows that reducing ADAR1 promotes axonal outgrowth.

The paper characterizes the immunoglobulin lambda chain locus in diverse populations, identifying novel alleles and highlighting limitations of short-read sequencing.

The paper generates bat induced pluripotent stem cells and finds they contain endogenous viral sequences, suggesting a deep entanglement between bats and viruses.

This study generates a large single-cell RNA sequencing dataset of the human substantia nigra in Parkinson's disease, identifies DA neuron subtypes, and reveals molecular changes associated with PD.

The paper identifies extracellular histones as new inhibitors of CNS axonal regeneration, showing they induce growth cone collapse and can be reversed by activated protein C.

The paper uses single-cell RNA sequencing to study atrial and ventricular myocardial cell differentiation, identifying lineage trajectories and effects of retinoic acid on cardiac development.

This study evaluates a polygenic risk score for breast cancer in women aged 70 and older, finding it improves risk prediction beyond conventional factors.

The paper identifies lipid-lowering genetic variants in older adults without CHD, showing their association with lower LDL-C and reduced statin use.

BayesDeBulk estimates immune and stromal cell composition from bulk proteomic and transcriptomic data, outperforming existing tools in synthetic and real data examples.

This study examines the effect of APOE genotypes and polygenic risk scores on dementia and cognitive decline in a longitudinal cohort of healthy older individuals, finding increased risks for APOE ε4 carriers and high PRS tertiles.

The paper identifies proinflammatory monocytes/macrophages associated with resistance to PD-1/PD-L1 blockade in urothelial cancer using bulk and single-cell RNA sequencing.

This study shows that the ApoE4 variant in mice leads to worse recovery and reduced neuronal sprouting after spinal cord injury compared to ApoE3.