Edward A. Lemke

This study shows phosphorylation of TDP-43's low-complexity domain shifts its self-assembly from macrophase separation to micellization, potentially protecting against pathological aggregation.

The paper develops a thermodynamic model that unifies phase separation and β-sheet-based aging in intrinsically disordered proteins, validated using nucleoporin-98 variants.

The paper shows FG-NUPs in live cells exhibit liquid-like mobility, while in vitro they transition to solid, with O-GlcNAcylation and importin-β maintaining the dynamic FG network's liquidity.

This study evaluates improvements in direct RNA sequencing (DRS) using RNA004 chemistry and modified-base-calling models, showing enhanced throughput, accuracy, and detection of RNA modifications in clinical applications.

This study examines the conformational changes of Nup98-HOXA9's FG domain across different assembly states, revealing micelle-like structures and dynamic transitions during condensate formation.

This study shows that PEG affects phase-separated droplets differently depending on the protein, accelerating Nup98 maturation but stabilizing BSA droplets.

This study shows that small heat shock proteins inhibit FUS and TDP-43 nano-cluster formation and target aggregation interfaces, highlighting their role in preventing neurodegenerative protein aggregation.