Sangeeta N. Bhatia

The paper develops scalable cell-free protein synthesis platforms to generate single-cell RNA-sequencing data on immune cell responses to cytokines and other signals, enabling functional discovery and drug target prioritization.

The paper evaluates a natural AAV capsid liver targeting motif in human hepatocytes using in vivo and in vitro models, showing its role in liver targeting and the impact of PEG on transduction.

The paper identifies caspase-1 as a target for lung cancer interception by analyzing protease activity in a murine model and showing reduced tumor formation with combined IL-1b and caspase-1 inhibition.

This study shows electrical stimulation promotes the formation of functional, perfusable vasculature in engineered tissues by inducing endothelial cell hyperpolarization via KV channels.

This paper develops a 3D in vitro model of the human hepatobiliary junction that recapitulates bile flow and dysfunction under hypoxia-reoxygenation, offering insights into biliary pathophysiology.

This paper develops a method to grow engineered liver tissue in vivo by using synthetic biology to trigger on-demand expansion after implantation.

The paper develops INSITE, an injectable platform for hepatocyte transplantation that enables precise delivery and long-term function through image-guided assembly into vascularizable scaffolds.

This paper identifies molecular features of monoclonal antibodies that enhance ctDNA detection by increasing its concentration in blood.

This study identifies Wnt9b-Sfrp2 crosstalk between endothelial and stellate cells as a driver of fibrosis resolution in MASH using single-cell and spatial profiling.

CleaveNet is an AI pipeline that designs protease substrates with high efficiency and selectivity, validated experimentally for MMP13.

This paper introduces a dual strategy to improve antimicrobial safety by engineering conditionally-active drug conjugates and co-administering antidotes, reducing toxicity while maintaining efficacy against bacterial infections.

This study uses single-cell RNA sequencing to map Plasmodium transitions and interactions in Anopheles mosquitoes, identifying key molecular processes and potential targets for transmission-blocking strategies.

This study designs nanobody-targeted conditional antimicrobial therapeutics to deliver therapeutic cargos to bacterial infection sites by enhancing protease activation through proximity-enabled reactivity.

This paper engineers multiplexed synthetic breath biomarkers to detect disease-specific protease activities and applies them to diagnose lung cancer and monitor treatment response in mice.

This study develops a scalable method to expand primary human hepatocytes while preserving their function, enabling improved liver cell therapy.

This paper develops C-FLIPs, protease-activated liposomes that deliver macromolecular therapeutics to the cytosol of cancer cells, inducing tumor regression and immune memory.

This study shows that different FGFR2 mutations in cholangiocarcinoma affect biliary architecture based on the epithelial state of the cells, revealing distinct phenotypic consequences.

This paper introduces a point-of-care diagnostic platform for lung cancer using inhalable nanosensors that detect tumor-associated proteases through urine analysis.

A DNA-binding agent increases cfDNA levels in blood, improving ctDNA detection and cancer sensitivity in mice.

A nanoparticle priming agent increases cfDNA levels in blood, improving ctDNA detection sensitivity in tumor-bearing mice.

The paper introduces PAA, a toolkit for analyzing protease activity data, offering preprocessing, visualization, and machine learning tools to standardize analysis across in vitro and in vivo assays.

This paper develops genetically encoded magnetic nanoparticles for tumor imaging by incorporating pH-sensitive and targeting peptides, showing enhanced binding and MRI contrast in vitro and in vivo.

This study uses activity-based nanosensors to detect early-stage lung cancer in mice with high sensitivity and specificity through urinary readouts of protease activity.