Spencer A. Haws

This study introduces CRISPRm, a dCas9-metabolic enzyme fusion platform, to investigate how nuclear metabolic perturbations affect gene expression and chromatin states in HEK293T cells.

The paper shows methionine, not SAM, is the primary metabolic signal for chromatin remodeling under stress, with methionine depletion causing epigenetic and transcriptional changes independent of SAM levels.

The paper investigates how dietary restriction of specific essential amino acids affects metabolism and chromatin states in mice, finding distinct effects for methionine, leucine, and isoleucine.

The paper shows that low H3K79 methylation in ESCs promotes transcription elongation at housekeeping genes, and inhibiting DOT1L enhances iPSC reprogramming by increasing H3K9ac.