Thomas Trian

ZIP CRISPR improves homology-directed repair efficiency by delivering ssODN templates via a heteroduplex gRNA-ssODN system, achieving up to 12-fold higher HDR editing in multiple cell types.

The paper introduces a bronchioid model using human bronchial stem cells in an alginate scaffold to mimic distal airway structure and function, showing ciliary activity and pathophysiological features.

This study quantifies fibrocyte density in bronchial tissue of COPD patients and controls, finding increased fibrocytes associated with reduced lung function and increased bronchial wall thickness.