Austin G Smith

This paper introduces cESFW for scRNA-seq analysis, revealing high-resolution developmental trajectories in human embryos and identifying lineage-specific markers.

This study shows ERK signalling dismantles the naive pluripotent state and preserves pluripotency during transition to formative pluripotency in mouse embryonic stem cells.

This study identifies YAP suppression as the mechanism by which tankyrase inhibition maintains human naive pluripotency without inducing differentiation.

Entropy Sorting identifies meaningful cellular heterogeneity in scRNA-seq data, revealing the inner cell mass in human pre-implantation embryos.

The paper shows that disabling de novo DNA methylation in embryonic stem cells causes them to adopt a trophoblast fate instead of proper differentiation, due to misexpression of Ascl2.

The paper shows human naive epiblast cells can generate trophectoderm and trophoblast cells, unlike mouse embryonic stem cells, and identifies key regulators of this process.

The paper identifies and characterizes formative stem cells in mice and humans, showing they have unique transcriptional and epigenetic features compared to naive and primed pluripotent cells.

The paper tracks mouse embryonic stem cells during differentiation, finding symmetric divisions and variable lag periods before loss of pluripotency.

This paper demonstrates that tankyrase inhibition enables efficient, transgene-free reprogramming of human somatic cells to naive pluripotency, with clonal expansion and differentiation capabilities.

This study identifies a common molecular logic governing embryonic stem cell self-renewal and reprogramming through Boolean network analysis and experimental validation.

This study compares single-cell transcriptomes of human, marmoset, and mouse embryos to identify conserved and divergent features of preimplantation development.

Reanalysis of three human embryo scRNA-seq datasets resolves cell-type ambiguities, defines epiblast and primitive endoderm prototypes, and identifies t2iLGö and 5i/L/A naïve stem cells as epiblast-like.

This study uses CRISPR/Cas9 to edit rat embryonic stem cells, generating in vitro models and in vivo reporters for genetic studies.

This paper describes a method to reset human pluripotent stem cells to a naive state through transient histone deacetylase inhibition, showing transcriptomic and epigenomic changes similar to the inner cell mass.

This study shows that Nodal maintains pluripotency in mouse ES cells as they exit the ground state, enabling competence for germline and somatic lineage specification.

The paper tracks the transition of mouse embryonic stem cells from ground state pluripotency to a formative phase, showing acute exit from naive status and transcriptome changes similar to early post-implantation epiblast.