Renier A. L. van der Hoorn

This study shows that NbRD21 protease regulates receptor-like kinase homeostasis in Nicotiana benthamiana by affecting their post-translational processing and endocytic recycling.

The paper introduces polychrome activity profiling using fluorescent probes to distinguish Cys proteases and their isoforms in plants by exploiting differential probe affinity.

This study identifies Cpi1, a membrane-anchored protease inhibitor in Pseudomonas that inhibits plant PLCPs, enhancing bacterial root colonization by modulating immune responses.

This study shows that eliminating polyphenol oxidase in Nicotiana benthamiana improves recombinant protein yield, purity, and native-state integrity during extraction.

This study identifies a bacterial outer membrane protein as a substrate of a citrus protease, showing that protease activity reduces pathogen titers and delays disease symptoms in citrus.

This study shows aphid effector proteins recruit plant immune regulators to processing bodies, suppressing defenses, while a plant protein counteracts this effect.

This study shows that silencing polyphenol oxidase in Nicotiana benthamiana reduces enzymatic browning and protein crosslinking without affecting plant growth.

This study uses reactivity proteomics to analyze ATP-induced changes in Arabidopsis proteomes, revealing conformational shifts and metabolite-protein interactions.

This study identifies galactosyrin, an iminosugar produced by Pseudomonas syringae, which inhibits plant β-galactosidase and alters host glycobiology to aid infection.

The paper identifies immunity genes that suppress transient protein expression in Nicotiana benthamiana by silencing them and observing increased GFP expression.

The paper shows that the subtilase SBT5.2 cleaves flagellin's flg22 epitope, inactivating its immunogenicity and controlling immune responses in plants.

The paper shows that the plant subtilase SBT5.2a cleaves the bacterial cold-shock protein csp22, reducing its immunogenicity and pathogen growth.

The paper evaluates AlphaFold Multimer's ability to predict interkingdom protein-protein interactions through in-silico screening of pathogen-secreted immune hydrolase inhibitors.

This study bioengineers proteases to convert Rcr3 orthologs and Pip1 into immune co-receptors that trigger Avr2/Cf-2 dependent immunity in tomato.

The paper shows that SBT5.2 subtilases degrade the IgG antibody 2F5 in Nicotiana benthamiana apoplast, and their knockout increases 2F5 accumulation without affecting plant growth.

The paper identifies active cytochrome P450s in mouse, plant, and fungal systems using chemical probes and mass spectrometry, showing their subcellular localization and activity-based labeling.

This study identifies flg22-responsive RNA-binding proteins in Arabidopsis, revealing their role in plant immunity and uncovering novel immune components.