Jeremy S. Dittman

The paper compares mammalian and C. elegans orthologs of Synaptotagmin-1 and Complexin in a single-vesicle assay to understand their roles in synaptic vesicle clamping and neurotransmitter release.

This study shows that two Munc13 oligomers are essential for vesicle docking and SNARE assembly, supporting neurotransmitter release.

iAChSnFR, a fluorescent indicator built from a bacterial periplasmic binding protein, reports acetylcholine with large, fast signals in mouse, fish, fly and worm preparations.