Paola Picotti

The paper shows that BAY-805 induces USP21-KLHL12 association, recruiting USP21 to COPII vesicles and impairing large cargo transport.

This study introduces QuPID-MS to measure protein phase separation in cells, showing weak interactions drive proteome demixing and influence environmental response.

A parallelized BindCraft pipeline generated 306,146 in silico binder candidates against 8,296 human proteins, each with sequence, predicted complex and confidence metrics, freely accessible online.

The paper identifies inhibitor-induced structural changes in kinases that drive non-catalytic effects, revealing the ATP-binding site as a key regulator of kinase conformation and interactions.

This paper develops a method to quantitatively analyze the structural states of alpha-synuclein in vitro and in situ, revealing conformational changes in yeast and human neurons.

This study identifies HPCAL1 as a Ca2+-dependent regulator of AC8, revealing a new mechanism for cAMP signaling regulation.

The paper identifies and quantifies in-source fragmentation (ISF) in proteomics data, showing its prevalence and impact, and proposes an open-source method to detect and remove ISF peptides.

This study identifies structural and functional differences in alpha-synuclein fibrils from Parkinson's disease, Dementia with Lewy Bodies, and Multiple System Atrophy, linking these differences to disease-specific cellular responses and potential therapeutic targets.

The paper introduces in-cell LiP-MS to study protein structural changes and biomolecular condensates in live human cells, revealing structural alterations under stress and validating them with microscopy.

This study systematically maps metal-binding sites in E. coli using Metal-LiP, identifies 424 candidate metal-binding proteins, and suggests functional roles for metal binding.

This study shows that respiration-induced gene expression and stress response pathways are critical for maintaining cytoplasmic properties and survival in yeast quiescence.

This study shows that SARS-CoV-2 main protease (Mpro) activates coagulation factors VII and XII, increasing plasma clotting probability by 2.5-fold.

The paper identifies two distinct binding sites for connexin channel inhibitors mefloquine and 2APB, showing they block channel function through different mechanisms.

The paper reports the cryo-EM structure of connexin-36 bound to mefloquine, revealing how small molecules inhibit gap junction channels by partially occluding the pore and altering electrostatics.

The paper introduces a framework to deconvolve LiP-MS signals and infer protein structural changes using data from three species.

This study systematically identifies structure-specific protein-protein interactions using LiP-MS, demonstrating its application to alpha-synuclein in Parkinson's disease.

This paper introduces a structural proteomic pipeline to study anti-amyloidogenic compounds' mechanisms in vitro and in situ, revealing off-target effects and structural interactions with α-Synuclein.

The paper identifies a central state transition in yeast molecular networks linked to PKA and TOR signaling, showing how genetic variants can cause global or local molecular changes affecting multiple cellular traits.

This paper introduces a data-driven method to detect functional proteoform groups from bottom-up proteomic datasets, identifying state-specific proteoform groups linked to molecular mechanisms.

This paper shows that ASC controls AA amyloidosis by interacting with SAA and that anti-ASC antibodies reduce amyloid loads in mice.

The paper uses single-cell proteomics and perturbation experiments to improve drug sensitivity predictions in breast cancer and identify genomic features associated with treatment response.

The paper identifies a natural variant in fission yeast Pyk1 that reduces glycolytic flux, increases respiration, and enhances oxidative-stress resistance but decreases growth.

LiP-Quant identifies drug targets in complex proteomes using limited proteolysis and mass spectrometry, providing EC50 estimates and distinguishing between homologous proteins.