David N. Nguyen

CRISPR-dPCR quantifies CRISPR knock-in by distinguishing integration from donor abundance and background, using multiplex digital PCR with orthogonal strategies.

This study compares LNP and electroporation for delivering CRISPR to HSPCs, finding LNPs provide better cell viability and HbF induction with lower toxicity.

This paper develops a lipid nanoparticle (LNP) platform for non-viral CRISPR-mediated homology directed repair (HDR) to generate CAR T cells with improved efficiency and reduced toxicity compared to electroporation.

The paper uses CRISPR to assess the functional impact of IL2RG variants in human T cells, confirming known annotations and identifying novel loss-of-function mutations.

This study identifies NKp30:B7-H6 and NKG2D:MICB/ULBP2 as receptor-ligand pairs that contribute to NK cell recognition of HIV-infected cells.

This paper introduces PERC, a method for efficient CRISPR editing in primary human immune cells and HSPCs using a peptide reagent, achieving over 90% editing efficiency without specialized equipment.

The paper develops a non-viral method using hybrid ssDNA repair templates to improve genome engineering in primary human cells, achieving high knock-in efficiency and cell yields for disease modeling and cell therapy.

The paper identifies CDC7 inhibition as a method to enhance HDR efficiency in CRISPR-Cas9 gene editing by inducing an early S-phase cell cycle arrest.

This paper develops a platform to discover synthetic DNA sequences that enhance T cell functions by integrating them into endogenous genomic sites.

The paper improves non-viral CRISPR-Cas9 genome editing in immune cells by using truncated Cas9 target sequences and polymer-stabilized nanoparticles, enhancing efficiency and viability.

This study shows that DNA double-strand breaks cause ribosome remodeling and translational shutdown, independent of p53 and preceding transcriptional changes.

This paper develops a non-viral CRISPR-Cas9 system for precise genome editing in human T cells, enabling therapeutic reprogramming of cell function and specificity.