Robert A. Holt

This paper introduces a de novo algorithm to reconstruct allele sequences from Oxford Nanopore amplicon reads, demonstrating its application to CYP2D6 and HLA with high accuracy.

This study compares multimerization domains in mRNA LNP vaccines, finding that IMX313 enhances antibody responses more than other domains.

The paper identifies novel DNA sequence motifs that modulate transcription in T cells using computational and experimental methods.

The paper uses Tope-seq to identify cross-reactive epitopes in an engineered T-cell receptor that caused off-target toxicity, highlighting the need for comprehensive screening.

This paper introduces a synthetic cytotoxic T cell platform for rapidly prototyping TCR function, demonstrating successful antigen-specific responses and preservation of antigen-presenting cells.

The paper describes a cost-effective and accurate plasmid sequencing method using the MinION device, achieving 100% accuracy in consensus sequences and detecting small mutations.

This study investigates how three Fusobacterium nucleatum strains modulate host cell transcriptomes and epigenomes through RNA-seq and ChIP-seq, revealing inflammation-related gene upregulation and chromatin remodeling during host cell invasion.

The paper reports the first draft genome of the North American bullfrog, providing genomic resources for amphibian research and supporting taxonomic classification.

The paper analyzes the interaction between tumor and immune clones in ovarian cancer, finding that immune contexture patterns mirror malignant spread and that foldback inversions correlate with poor outcomes.