Lei Jin

This study compares BBB-targeting AAV capsids for CNS delivery efficiency and safety, finding CNSRCV300 offers the best balance between brain targeting and biosafety.

This study uses barcoded rabies virus with single-cell and in situ sequencing to map synaptic connectivity in the mouse brain, distinguishing cortical cell types by their connectivity patterns.

The paper shows that a modified rabies virus intended for noncytotoxic neuronal tracing lost its modification due to mutations, leading to paradoxical results, and that the modified virus requires exogenous protease for efficient spread.

This paper introduces third-generation rabies viral vectors with single deletions that enable more efficient, non-toxic retrograde targeting of projection neurons compared to previous generations.

This paper introduces a non-toxic rabies virus system for long-term in vivo imaging of neuronal networks, showing minimal toxicity and normal visual response properties over 10 weeks.

The paper shows that helper virus concentrations significantly affect transsynaptic tracing efficiency and nonspecific labeling in monosynaptic tracing experiments.

The paper demonstrates the use of ChR2-XXL and GtACR1 in primary rat cortical neurons to manipulate neuronal firing patterns through high current optogenetic channels.