Valérie de Crécy‐Lagard

This study identifies SMU_393 as a homolog of RocS in S. mutans through phenotypic profiling of essential genes, revealing its role in chromosome segregation and cell cycle control.

This study identifies YhcC as an anaerobic alternative to MnmC for demodifying cmnm5s2U to nm5s2U in E. coli tRNA.

This study maps the functional diversification of the QueC protein family, identifying residues critical for tRNA modification and characterizing its repurposing in anti-phage defense and other biochemical pathways.

The paper integrates genome mining and structure-guided modeling to discover new 7-deazapurine-containing biosynthetic gene clusters and their chemical functions.

This study maps tRNA modifications in Streptococcus mutans and S. pneumoniae, revealing shared losses and clade-specific adaptations in modification genes and metabolic strategies.

This study characterizes McHemW, a heme-binding radical SAM enzyme in Moraxella catarrhalis, showing it binds heme and contains an active [4Fe-4S] cluster, suggesting a role in heme transport.

The paper evaluates machine-learning models' ability to predict enzymatic functions for uncharacterized proteins and finds they fail to integrate scientific reasoning and make logic errors.

The paper profiles tRNA modifications in Pseudomonas aeruginosa to uncover regulatory networks and validate gene annotations.

This paper identifies SLC35F2 as a high-specificity transporter for queuine and queuosine in multiple species and shows its role in regulating intracellular levels of these micronutrients.

The paper introduces GATOR-GC, a genome mining tool that identifies diverse gene clusters and maps their evolutionary patterns, revealing previously uncharacterized biosynthetic functions.

This study identifies genetic interactions among tRNA modification genes in E. coli K12, revealing synthetic lethal pairs and condition-dependent phenotypes.

This study identifies a unique evolutionary pathway in Bartonella henselae for queuosine salvage, showing it can use both preQ1 and q, unlike other pathogens.

This paper identifies a novel pathway for mnm5s2U synthesis in Gram-positive bacteria, revealing alternative biosynthetic mechanisms involving the MnmL enzyme.

The paper maps tRNA modifications and their synthesis genes in two Bartonella species, identifying gene reductions linked to reductive evolution.

The paper argues that AI platforms for predicting protein function risk overprediction and highlights the need for multiple evidence types, using an analysis of YciO protein function predictions in E. coli.

The paper identifies four new 7-deazaguanine derivatives in phages and characterizes enzymes involved in their biosynthesis and insertion into DNA.

The paper introduces AQRNA-seq, a method for accurate small RNA quantification, and demonstrates its application to bacterial tRNA pools revealing copy number variation and modification patterns.