Matteo Ciciani

The paper describes the development of En-ISPmu1, a modified TnpB nuclease, and demonstrates its ability to achieve targeted genome integration in mouse liver with a single AAV vector.

The paper enhances Cas{Phi}2 activity using EPICA.2, an optimized directed evolution platform, resulting in evoCas{Phi}2 with 70-fold increased activity in human cells.

This study uses CRISPR base editing to introduce revertant mutations in F508del-CFTR, rescuing protein folding and function in CF patient-derived cells.

This study enhances weakly active Cas9 nucleases using a eukaryotic platform, resulting in a 12-fold increase in activity in mammalian cells while maintaining specificity.

The paper presents a method to discover new human gut viruses using enriched virome samples and reports over 162,000 novel viral sequences.