Thomas Mangeat

This paper introduces optofixation, a method using light and DNA-binding fluorophores to rapidly fix live cells with intact nuclei and membranes.

This study introduces NLS-LARIAT, an optogenetic tool to cluster nuclear proteins in Drosophila ovarian cells, revealing their roles in migration and nuclear structure.

This study shows that goblet cells create tight junction fractures between enterocytes, increasing gut permeability under homeostatic conditions and during hypertrophy.

This paper introduces a method for absolute force measurements in living tissues with sub-piconewton precision, validated in Drosophila pupae and embryos.

This study identifies ST8SIA4-mediated polysialylation as essential for CCR2-driven monocyte egress from the bone marrow, revealing a novel regulatory mechanism in immune cell trafficking.

The paper identifies PERIOD proteins as regulators of TC-DSB repair by anchoring DSBs to the nuclear envelope, linking circadian rhythms to DNA repair mechanisms.

The paper investigates how the nucleus integrates mechanoprotection and mechanosensing during EMT by analyzing spatial patterns of chromatin response to mechanical forces.

This paper introduces 3D-RIM, a method that achieves super-resolved volume imaging of thick live specimens with high contrast and resolution across multiple microns.

This study characterizes the subcellular localization and nuclear import mechanisms of yeast RNA exosome subunits, revealing their nucleolar distribution and interactions with importins and cofactors.

EDF-RIM improves imaging speed and light dose while maintaining resolution for thick biological samples.

This paper introduces FlexSIM, a SIM reconstruction method that handles imaging challenges and achieves state-of-the-art performance on open datasets.

The paper shows that compressive stress during Drosophila leg development drives apoptosis through lateral tension and Piezo1, contributing to tissue folding.