jYCaMP: An optimized calcium indicator for two-photon imaging at fiber laser wavelengths

Manuel Alexander Mohr; Daniel Bushey; Abhi Aggarwal; Jonathan S. Marvin; Jeong Jun Kim; Emiliano Jimenez Marquez; Yajie Liang; Ronak H. Patel; John J Macklin; Chi-Yu Lee; Arthur Tsang; Getahun Tsegaye; Allison M. Ahrens; Jerry L. Chen; Douglas S. Kim; Allan M. Wong; Loren L. Looger; Eric R. Schreiter; Kaspar Podgorski

doi:10.1038/s41592-020-0835-7

NeuroscienceNature MethodsMay 25, 2020

Peer-reviewed journal article

jYCaMP: An optimized calcium indicator for two-photon imaging at fiber laser wavelengths

Manuel Alexander Mohr iD, Daniel Bushey iD, Abhi Aggarwal iD, Jonathan S. Marvin iD, Jeong Jun Kim iD, Emiliano Jimenez Marquez, Yajie Liang iD, Ronak H. Patel iD, John J Macklin iD, Chi-Yu Lee iD, Arthur Tsang iD, Getahun Tsegaye, Allison M. Ahrens, Jerry L. Chen iD, Douglas S. Kim iD, Allan M. Wong iD, Loren L. Looger iD, Eric R. Schreiter iD, Kaspar Podgorski iD

Abstract

State-of-the-art GFP-based calcium indicators do not undergo efficient two-photon excitation at wavelengths above 1000 nm, for which inexpensive and powerful industrial femtosecond lasers are available. Here we report jYCaMP1, a yellow variant of jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1000 nm and enables improved two-color calcium imaging with RFP-based indicators.

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