Advanced Fluorescence Microscopy Techniques

Intravital single-molecule microscopy reveals protein kinetics in live mammalian tissues

This paper introduces iSiMM, a method for tracking single molecules in live tissues, and demonstrates its application to study protein dynamics in mice.

Correlation of Live Imaging and Fixed-Expansion Microscopy to study intracellular pathogens

This study develops LiFEx to correlate live-cell imaging with expansion microscopy, revealing membrane changes linked to bacterial dissemination.

A Chemigenetic Fluorescence Lifetime Biosensor for Dopamine Sensing

This paper introduces dHaloLife635, a chemigenetic fluorescence lifetime sensor for dopamine that shows selectivity and stability in live cells.

EverGreen: A Calcium Sensing Fluorescent Protein with Ultra-Slow Response Properties

The paper introduces a fluorescent calcium biosensor with ultra-slow response kinetics, useful for applications where delayed signal detection is advantageous.

Design Tradeoffs in High Resolution, Large Field-of-View 3D All-Optical Control of Neural Circuits

The paper explores tradeoffs between resolution and field of view in scanless temporally focused holographic optogenetic systems, demonstrating improved spatial control using high NA objectives.

Open Blink: Low-cost TIRF microscopy for super-resolutionimaging via μManager

Open Blink provides a low-cost, open-source TIRF microscope for quantitative multi-color super-resolution imaging with high localization precision and axial stability.

NanoBlocks: creating fluorescent biosensors from affinity binders using competitive binding

This paper introduces NanoBlocks, a method to convert affinity binders into fluorescent biosensors by adding competitive peptides, demonstrating their use in detecting targets in vitro and in cells.

Multi-region two-photon calcium imaging in freely moving animals

This paper introduces MINI2P-II, a lightweight 2P miniscope enabling multi-region calcium imaging in freely moving animals with high resolution and dual-color capability.

Quantitative imaging of calcium dynamics with a green fluorescent biosensor and fluorescence lifetime imaging

The paper describes protocols for calcium imaging using a genetically encoded biosensor and fluorescence lifetime imaging to improve measurement robustness.

Ratio-preserving super-resolution FLIM for quantitative live-cell lifetime imaging

This paper introduces SIM-fdFLIM, a super-resolution FLIM method that preserves ratio accuracy and enables high-speed, high-resolution live-cell lifetime imaging.

Bayesian in situ point spread function modeling enables high-fidelity three-dimensional nanoscopy in deep tissue

This paper introduces Bayesian in situ PSF modeling to improve 3D nanoscopy in deep tissue by accounting for optical distortions and uncertainties in PSF estimation.

Multiscale, Survey-and-Target Optical Super-Resolution Histology

This paper introduces MOSH, a multiscale optical super-resolution histology platform that enhances resolution while maintaining tissue-scale context and throughput.

Quanta microscopy enables fluorescence imaging at the native bit-plane rate

Quanta microscopy enables high-speed fluorescence imaging at the native bit-plane rate by reconstructing multibit images from sparse photon-detection maps.

Cellular-resolution Three-photon Ultra-Deep Imaging (TrUDI) of neuronalpopulation activity at 2 mm depth in the mammalian brain

The paper introduces TrUDI, a three-photon microscopy system that improves imaging depth in the mouse brain to ~2 mm with enhanced fluorescence detection.

In situ pulse dispersion estimation via third-harmonic generation interferometric autocorrelation for multiphoton microscopy

This paper develops a method to estimate pulse dispersion in multiphoton microscopy using third-harmonic generation interferometric autocorrelation, demonstrating its effectiveness in vivo.

Continuous mass photometry by single molecule trapping

This paper introduces a method for continuous mass photometry of single molecules, enabling long-term observation of biomolecular interactions with high mass precision and temporal resolution.

Physics-driven self-supervised learning for quantitative high-fidelity structured illumination microscopy

This paper introduces qHiFi-SIM, a physics-driven self-supervised learning framework for quantitative high-fidelity structured illumination microscopy, achieving excellent intensity linearity and structural fidelity without ground-truth data.

Vision Transformers as a Framework for Imaging Fluorescence Correlation Spectroscopy

The paper introduces ImFCSViT, a Vision Transformer framework that improves fluorescence correlation spectroscopy by directly analyzing raw image data and extracting biophysical parameters more efficiently than traditional methods.

iFLiP: accessible and low-cost lifetime fiber photometry for robust longitudinal and quantitative measurements of neural dynamics

Organelle-Specific pH Quantification Using a Protein-Based Quantum Sensor

This study characterizes MagLOV2, a genetically encoded quantum sensor, showing its organelle-specific localization and pH-dependent response kinetics, with applications in tracking mitophagy and acidification.

Steric attenuation of membrane-associated dimerization-induced fluorescence for cell-free biosensing

This study develops a cell-free biosensing reporter using rapalog-induced split sfCherry reconstitution and demonstrates steric attenuation of fluorescence by antibodies binding to epitope tags.

Super-resolution imaging flow cytometry

This paper introduces super-resolution imaging flow cytometry (SR-IFC) to quantify organelle architecture heterogeneity at population scale, achieving sub-diffraction resolution in microbiology and immunology applications.

RhoBAST-FLIM enables high-fidelity and lifetime-assisted super-resolution RNA imaging

RhoBAST-FLIM improves RNA imaging by using fluorescence lifetime differences to enhance contrast and resolution in live cells.

A simple platform for in situ DNA-PAINT optimization enables improved multiplexed super-resolution imaging in Gram-negative bacteria

The paper develops a platform for optimizing DNA-PAINT in Gram-negative bacteria, enabling high-quality 3D imaging of multiple targets in E. coli and Yersinia enterocolitica.

Quantitative fluorescence lifetime imaging of protein microenvironments by genetically encoded compact benzodiazole probes

This paper introduces genetically encoded benzodiazole-based probes for quantitative FLIM, enabling site-specific analysis of protein microenvironments in living cells.

Localization accuracy in single-molecule fluorescence microscopy

This study compares the accuracy of two single-molecule localization methods in microscopy, finding that MINFLUX significantly outperforms camera-based methods.

Fiber-Coupled Rotating Two-Photon Light-Sheet Microscopy for Physiological Vestibular Imaging in Larval Zebrafish

This paper introduces a rotating two-photon light-sheet microscope for vestibular imaging in larval zebrafish during physiological stimulation.

Adaptive-optics lattice light-sheet microscopy enables correlative single-molecule imaging of endogenous proteins in native environments

ALPINE enables 3D single-molecule imaging and tracking of endogenous proteins in native environments with high resolution and dynamic quantification.

Cortex-wide single-cell activity mapping in freely moving mice

The paper introduces COMET, a lightweight head-mounted mesoscope for cortex-wide single-cell calcium imaging in freely moving mice, demonstrating its ability to capture neural activity during natural behaviors.

Red fluorescent labeling of myelin by membrane-targeted tdTomato in transgenic mouse lines

The paper develops transgenic mouse lines with red fluorescent labeling of myelin, enabling structural and functional imaging compatible with green spectral sensors.