MAGIC: A label-free fluorescence method for 3D high-resolution reconstruction of myelinated fibers in large volumes

Irene Costantini; Enrico Baria; Michele Sorelli; Felix Matuschke; Francesco Giardini; Miriam Menzel; Giacomo Mazzamuto; Ludovico Silvestri; Riccardo Cicchi; Katrin Amunts; Markus Axer; Francesco Saverio Pavone

doi:10.1101/2020.07.28.225011

NeurosciencebioRxivJul 28, 2020

Preprint

MAGIC: A label-free fluorescence method for 3D high-resolution reconstruction of myelinated fibers in large volumes

Irene Costantini iD, Enrico Baria iD, Michele Sorelli iD, Felix Matuschke iD, Francesco Giardini iD, Miriam Menzel iD, Giacomo Mazzamuto iD, Ludovico Silvestri iD, Riccardo Cicchi iD, Katrin Amunts iD, Markus Axer iD, Francesco Saverio Pavone iD

Abstract

Analyzing the structure of neuronal fibers with single axon resolution, in large volumes, remains an unresolved challenge in connectomics. Here, we propose MAGIC (Myelin Autofluorescence imaging by Glycerol Induced Contrast enhancement), a simple tissue preparation method to perform label-free fluorescence imaging of myelinated fibers. We demonstrate its broad applicability by performing mesoscopic reconstruction at sub-micron resolution of mouse, rat, monkey, and human brain samples and by quantifying the different fiber organization in Control and Reeler mouses hippocampal sections.

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