Antimicrobial Resistance in Staphylococcus

This study uses ATP-interacting protein profiling to analyze S. aureus-host interactions in macrophages and keratinocytes, revealing cell-type specific adaptations and metabolic shifts.

This study identifies SasG-II, a variant of the S. aureus surface protein, which binds to a broader range of ligands, enhancing its ability to colonize skin.

This study develops a cost-effective media to mimic the human skin environment and shows it enhances the expression of virulence factors in MRSA.

This study identifies that mutations in PBP4 and GdpP synergistically confer high-level, broad-spectrum β-lactam resistance in mecA-deficient Staphylococcus aureus strains.

This study identifies Desemzia incerta as a skin commensal that reduces MRSA colonization through secreted antimicrobial proteins and decreased biofilm formation in a murine model.

This paper predicts S. aureus antimicrobial resistance using interpretable genomic space maps generated by generative topographic mapping (GTM) applied to genomic data from the PATRIC database.

This study uses NMR spectroscopy to determine the substrate specificities of lysostaphin and LytM, showing they prefer D-Ala-Gly cross-linked parts of peptidoglycan, with LytM also acting as a D-alanyl-glycine endopeptidase.

The paper uses TDbasedUFE to analyze scATAC-seq data from S. aureus sepsis patients, identifying genes with differential responses between methicillin-resistant and sensitive strains and their functional associations.

The paper shows nasal commensals reduce S. aureus growth by consuming its siderophores, revealing inter-species competition and potential for probiotic strategies.

This study investigates diacerein's antimicrobial mechanism against MRSE and shows it can reverse β-lactam resistance by affecting efflux systems and inducing cell lysis.

This study characterizes neonatal Staphylococcus capitis NRCS-A isolates and compares them with non-NRCS-A isolates from neonates and adults, identifying genetic and phenotypic traits associated with their success in NICUs.

This study shows that 5-Fluorouracil inhibits Staphylococcus aureus growth and interacts with antibiotics, potentially selecting for antibiotic resistance.

The paper identifies a new family of chromosomal islands shared between Staphylococcus agnetis and Staphylococcus aureus that may contribute to pathogenicity in chickens.

The paper reconstructs the pre-epidemic evolution of the USA300 MRSA lineage using genomic data and identifies a third major clade and molecular markers for classification.

The paper uses nanopore sequencing to analyze the genome of a human-associated MRSA strain, identifying antibiotic resistance genes, plasmids, and prophages, and compares it to Pakistani MRSA isolates.

The paper describes a novel repression-derepression mechanism for Staphylococcus aureus pathogenicity islands (SaPIs) involving tetrameric repressors and an inducing protein.

The paper uses WGS to analyze 508 S. aureus isolates from India, identifying three major clones and two novel MRSA sub-lineages of CC22, with evidence of global transmission.

This study identifies genome degradation and convergent mutations in Staphylococcus aureus during severe infections, linking them to adaptation and clinical outcomes.

The paper develops a coevolution score method to detect coevolving genes in bacterial genomes, finding coordinated gene gain/loss patterns in S. aureus related to antibiotic resistance.

This study introduces Sketchy, a genomic neighbor typing tool for bacterial outbreak surveillance, showing improved lineage prediction but limitations in clade-specific genotype resolution.

The paper systematically evaluates key parameters affecting SNP-based transmission inference of MDROs, finding that using a closely-related reference genome and sliding-window analysis improves accuracy.

This paper uses nanopore sequencing and Random Forest models to estimate transmission dynamics of MRSA outbreaks, showing sustained transmission with Re > 1.

The paper sequenced S. aureus isolates from the Philippines to analyze MRSA clones and resistance patterns, finding a limited number of genetic clones and high concordance between phenotypic and genotypic resistance.

This study quantifies the modes of vanA-type vancomycin resistance dissemination in Dutch hospitals, finding clonal spread as the dominant mechanism with significant contributions from horizontal gene transfer.

The paper isolates and characterizes a novel Staphylococcus species from Mnemiopsis leidyi gut, distinguishing it from known species using MLSA and ANI.

This paper identifies MGB-BP-3 as an antibiotic that inhibits promoter isomerisation in S. aureus, showing resistance evolution is unlikely.

The paper evaluates GWAS methods for detecting antibiotic resistance in Staphylococcus aureus genomes, finding they identify novel resistance mutations but are less accurate than literature-based approaches.

The paper presents a Bayesian model to estimate bacterial acquisition and clearance rates by accounting for within-host genetic variation and sampling intervals, validated using simulations and applied to MRSA isolates.

This study compares the pathogenicity of Staphylococcus aureus and Streptococcus pyogenes isolates toward human myoblasts and keratinocytes, finding higher internalization and cytotoxicity by NSTI-SA in myoblasts.