Advanced Fluorescence Microscopy Techniques

This paper introduces SpinFlux, a modulation-enhanced spinning disk confocal microscopy method that improves localization precision over SMLM with minimal hardware modifications.

TARDIS provides a parameter-free alternative to single-particle tracking by analyzing temporal distance distributions to infer particle movement and interactions more robustly than existing methods.

PyF2F measures fluorophore-to-fluorophore distances from PICT data, validated on a published dataset.

The paper develops synthetic binders specific to PSD-95 for imaging endogenous proteins with minimal impact on function and compatibility with super-resolution techniques.

The paper introduces a method to map individual sensory nerve axons from digits to spinal cord at sub-micron resolution.

This study demonstrates the feasibility of 4- and 5-color fluorescence imaging in Dictyostelium discoideum using new fluorescent proteins and labels.

nanoFeatures is a cross-platform tool for quantitative analysis of super-resolution microscopy images of nanoparticles, extracting features like size, shape, and molecular abundance.

This paper develops an ultrahigh-throughput SMLM methodology using a color glass filter to achieve sub-10 nm localization and sub-5 nm spectral precision for multicolor imaging.

The paper develops a four-color single-molecule imaging system using fluorescent HiBiT peptides to track GPCR dynamics and interactions in living cells.

The paper describes the design of a super-resolution fluorescence cryomicroscope for single molecule localization microscopy of cryosamples.

This paper optimizes light-sheet fluorescence microscopy illumination beams using deep learning to improve cell detection performance.

This paper demonstrates how deep UV wavelengths can recover STED bleaching and enable reactivation of fluorophores for correlative STED and SMLM imaging.

This paper introduces a high-speed 3DSIM system using DMD and electro-optic modulators, achieving improved resolution and imaging speed for various biological specimens.

This study evaluates how the tilt of a coverslip affects STED and two-photon microscopy image quality and proposes a method to correct the tilt.

The paper introduces a long working distance air objective for multiphoton imaging in vivo, demonstrating its performance across multiple species and imaging systems.

The paper introduces a new fluorescent protein, LSSmApple, and uses it to create a genetically encoded calcium sensor for yeast imaging.

This paper introduces a far-red dye that selectively labels acidic organelles in live cells, enabling multicolor, time-lapse, and super-resolution imaging with improved photostability and compatibility with long-term experiments.

This paper introduces a new imaging technique to achieve brain-wide neuronal activity recording in adult vertebrates.

This paper introduces CNNs to improve Imaging FCS by reducing data needs and enabling real-time analysis.

The paper uses spectroscopy and X-ray structures to reveal two distinct mechanisms by which flavin-binding proteins tune their fluorescence spectra.

This paper introduces OT-TP-LSM for 3D pathology, achieving high imaging depths and enabling virtual H&E image generation via deep learning.

The paper develops improved GRABDA sensors to monitor dopaminergic activity in vivo, showing high sensitivity and spatially resolved DA release in mice during behaviors.

This paper introduces a method to image multiple dynamic signals in living cells using temporally multiplexed imaging with fluorescent proteins.

This paper introduces OPSIM, a structured illumination microscopy technique that enables rapid whole-cell imaging with doubled resolution, achieving up to 2.7 Hz for mitochondrial and ER dynamics.

This paper introduces a method to reduce speckle noise in multiplane HiLo microscopy using non-local means denoising, enabling fast, high-contrast volumetric imaging.

The paper develops GRAB sensors for detecting neuropeptides with high specificity and sensitivity, enabling in vivo monitoring of their release and function.

This paper introduces a UVA hyperspectral light-sheet microscopy method to map metabolic activity in 3D, demonstrating its application to pre-implantation embryo development.

The paper introduces exNEEMO, a method to quantify spectrally overlapping fluorophores using intra-exposure excitation modulation, enabling accurate per-pixel unmixing.

This study introduces PS-ExM, a modified expansion microscopy protocol for visualizing Plasmodium liver stage, offering faster processing and better epitope preservation.

This paper demonstrates that pulse gating improves signal generation and signal-to-noise ratio in two-photon microscopy for deep brain imaging in mice.