Glycosylation and Glycoproteins Research

GLYCO-2 improves computational efficiency for quantifying glycan shielding of glycoproteins, revealing increased shielding in influenza HA proteins over time.

The paper identifies glycan structures on flagellin proteins of Aeromonas hydrophila, revealing metaheterogeneity and a novel pseudaminic acid derivative.

This paper characterizes the glycosylation of soluble CD52 and identifies its role in immune suppression through interactions with HMGB1 and SIGLEC-10.

This study identifies miRNAs that regulate CMAS expression, impacting sialylation levels and showing bidirectional miRNA regulation in pancreatic cancer.

The paper identifies a specific O-glucose glycan extension on NOTCH1 EGF10 that impacts ligand binding and signaling, revealing new enzymatic pathways and structural features of Notch glycosylation.

The paper compares glycosylation patterns of IgA1 and IgA2, finding subclass-specific differences despite similar glycosylation enzyme expression.

The paper presents methods for glycan fingerprinting of therapeutic antibodies, identifying specific glycan structures on NISTmAb.

The paper uses a human lectin array to rapidly screen oligosaccharides for targeting specificity, validating it with existing ligands and identifying new candidates for cell-specific targeting.

This study identifies N-acetyl-β-D-hexosaminidase as a driver of colorectal cancer progression and a prognostic marker in patient plasma.

This paper develops specific antibodies to detect O-fucose on nucleocytoplasmic proteins in protists, slime molds, and plants, revealing conservation of the O-fucome across species.

The paper investigates how glycosylation and splicing variants of LRIG1 affect EGFR proteostasis in cancer, finding that N74Q mutation and exon 2 skipping enhance LRIG1-EGFR binding and degradation.

This study investigates the conformational flexibility of PMM2 using structural and simulation analyses, revealing dynamic states that inform pharmacological chaperone design.

This study investigates how MUC2 expression affects immune infiltration in colorectal cancer using in vitro models and CRISPR knockout, finding that MUC2 loss increases immune infiltration only in HT-29 cells.

This paper introduces Glyco-PAINT-APP, a method to quantify single-molecule glycan-mannose receptor binding kinetics on myeloid cells, revealing subcellular binding heterogeneity.

This study identifies the unknown moiety in the b-type flagellin glycan of Pseudomonas aeruginosa PAO1 as N,N-dimethylthreonine using mass spectrometry and compares it to Type A glycan in C. difficile.

This paper develops a mass spectrometry-based method to decode glycoproteins by integrating deglycosylation and glycosylation site characterization, enabling accurate sequencing of complex glycosylated proteins.

The paper shows that cell membrane glycan content creates a kinetic barrier to viral uptake in a protein-nonspecific way by forming a physical obstacle to viral adhesion.

This study develops a nanobody-enzyme fusion protein targeting PD-L1 and sialic acid, showing anti-tumor effects by modulating tumor-associated macrophages.

The paper shows polysialic acid (polySia) is present in immune cells and linked to activation, with sex-dependent increases in smokers.

This study characterizes the N-glycan structures and their impact on the structural dynamics and functional properties of the Abdala COVID-19 vaccine.

The paper uses lectin microarrays to analyze glycoproteins in salivary glands of Aedes aegypti and Anopheles stephensi, finding similar glycan profiles with specific oligomannose and O-glycan presence.

This study shows that reducing OGT activity in mice causes embryonic lethality proportional to the reduction in O-GlcNAc modification, with milder reductions causing developmental delays and retrovirus activation.

This study synthesizes sialylated and fucosylated mucin analogs to investigate glycan effects on protein conformation and degradation.

The paper identifies O-GlcNAcylation of DDX3X as a regulator of the G1/S-phase transition and shows its role in cell cycle control and neurogenesis in OGT-CDG.

The paper identifies and characterizes a fucose-binding lectin from Enterobacter cloacae with high specificity for LewisA and blood group O antigens.

The paper uses ZenoTOF mass spectrometry to analyze glycoproteomes from breast cancer and control samples, identifying 15 site-specific glycans as potential biomarkers for breast cancer detection.

This study compares N-glycosylation profiles of iPSCs and NSCs from DS and non-DS individuals, finding altered glycan structures in T21 cells during neural differentiation.

This paper introduces a framework for automated glycan nomenclature conversion that accepts any input format and outputs a standardized IUPAC-condensed format, enabling seamless integration of glycan data.

The paper reports a cryo-EM structure of Chlamydomonas mastigoneme, revealing a 5',5'-phosphodiester bond stabilizing glycan folding.

This study reveals the glycocalyx as an ordered, self-assembled hierarchical micro- and nanoarray structure conserved across evolution in human and sponge cells.