Glycosylation and Glycoproteins Research

This study develops an integrated mass spectrometry strategy to quantify the proteoform diversity of O-glycoprotein osteopontin, revealing systematic underestimation of glycan sialylation by bottom-up methods.

This paper develops an mRNA-based platform to transiently control sialoglycan biosynthesis on human cells by delivering sialyltransferase enzymes, showing increased surface presentation of specific sialoglycans with minimal off-target effects.

This paper discovers non-carbohydrate inhibitors of Siglec-7:GD3 interactions using BiGEL libraries and validates their potency through SPR, ELISA, and NMR.

This study shows that a human-like sialome increases susceptibility to metabolic stress in colorectal cancer, using a mouse model with Cmah deficiency and Apc mutation.

The paper shows that GALA, a process relocating GALNTs to the ER, enables co-translational O-glycosylation in pancreatic cancer, expanding the O-glycoproteome and promoting tumor growth.

The paper identifies soluble sialic acid as a conserved biomarker of inflammation through metabolomics in mouse tissues and cultured macrophages.

The paper defines the molecular basis of sequence-dependent N-glycosylation by human OST using simulations and experiments, confirming rules at proteome scale.

The paper develops an mPBA enrichment workflow to analyze N-glycoproteome remodeling under ER stress, revealing dynamic changes in glycosylation sites and glycoforms.

This study identifies S-palmitoylation of OGT and shows it stabilizes OGT by preventing CMA degradation and enhances its interaction with PPP1CC and YAP.

This paper introduces OGTAC, a molecule that enhances c-Myc O-GlcNAcylation, rewires its transcriptional activity, and inhibits cancer cell proliferation.

This study shows that transiently increased cardiomyocyte O-GlcNAcylation worsens cardiac remodeling after pressure overload, supporting the concept of O-GlcNAc memory as part of metabolic memory.

GlyComboCLI assigns glycan compositions to mass spectrometry data through a command-line interface, supporting diverse parameters and integration into FAIR workflows.

This study investigates the role of Lewis X (LeX) glycans in cancer cell evasion of NK cell-mediated destruction, finding that LeX has a limited impact compared to sialylated glycans.

This paper introduces a high-resolving power DIA-PTCR method for glycoproteoform analysis, enabling isotopically resolved mass measurements and integration with other glycoproteomics approaches.

This paper introduces DOGTACs, a method to selectively remove O-GlcNAc from target proteins using endogenous OGA in living cells, showing concentration- and time-dependent de-O-GlcNAcylation without affecting global O-GlcNAc levels.

The paper develops an enrichment-free glycoproteomics method using mass defect-driven glycopeptide classification to identify sex differences in murine plasma fucosylation.

This study identifies ST6GAL1-mediated sialyl linkage switching in TAMs that drives cancer-promoting nanotubes with α2,6-sialylation, affecting immune interactions and CRC progression.

This study analyzes N-glycans in dried blood spots (DBS) from different blood preparations to distinguish pre-diabetes and diabetes, showing trends in fucosylation, bisection, galactosylation, and sialylation.

The paper uses spatial glycomics to show that N-glycan abnormalities in MOGHE are linked to hypomyelination and are more pronounced in pediatric cases.

The paper shows that specific N-glycan profiles in extravillous trophoblasts (EVTs) are critical for maintaining immunological protection against natural killer (NK) cell cytotoxicity.

This study shows that altering N-linked glycan terminal sialylation on K562 cells increases their susceptibility to NK-92 cell-mediated cytolysis.

The paper identifies a glycan phosphatase function of dystroglycan that regulates matriglycan extension by removing phosphate from xylose.

This study uses GlycoSHIELD modeling and DNA-PAINT SMLM to show that N-glycosylation promotes Na,K-ATPase clustering by forming a steric shield, supporting a galectin-lattice organization mechanism.

Desialylation enhances bispecific T cell engager responses by relieving a sialoglycan-dependent restraint in human T cells.

This paper introduces GPMAW glyco-search, a workflow for high-confidence identification of intact sialylated N-glycopeptides using TiO2 enrichment, dual LC-MS/MS, and interactive spectrum validation, achieving over 2800 identifications in human plasma.

This study identifies an O-GlcNAc modification on PKM2 that promotes PDAC progression through the ARNT-CDC27-AKT pathway by enhancing aerobic glycolysis.

This study shows that STn-positive tumor-derived extracellular vesicles impair dendritic cell function by transferring glycans, leading to immunosuppression.

This study shows Salmonella uses 2,3-sialylated MUC1 on colonocytes for apical invasion, identified through glycan analysis and ex vivo models.

The paper evaluates how glycan-binding proteins and immune cells recognize mirror-image glycans, finding widespread binding and donor-specific recognition of L-glycans.

The paper uses high-speed AFM and biophysical methods to study dynamic conformational changes in multidomain glycosyltransferases, revealing diverse organizational strategies.