Glycosylation and Glycoproteins Research

GlycansToGraphs is a tool that identifies and visualizes mass relationships in glycopeptide spectra without requiring databases.

This study removes O-GlcNAc from mouse preimplantation embryos and finds it slows embryonic growth without affecting genome activation.

This study presents the first large-scale IgA glycomics dataset from twins, identifies shared and specific genetic loci influencing IgA and IgG glycosylation, and shows genetic conservation across ethnicities.

This study reveals the substrate recognition and specificity mechanisms of Alg1 using molecular dynamics simulations and mutant assays.

The paper identifies dysregulation of PLOD3 in oligodendrocytes as an upstream regulator of COL4A5, linked to amyloid fiber formation in Alzheimer's disease.

This study identifies specific N-glycans on Notch1 receptor that regulate Jagged-1 induced Notch signaling and myogenic differentiation of vascular stem cells.

The paper develops an improved N-glycoproteomic workflow to analyze low-abundant glycoproteins in human blood plasma, enabling the identification of 1,929 N-glycopeptides and 942 N-glycosites.

This study investigates OGT-CDG using stem cells, finding disrupted O-GlcNAc homeostasis and morphology during neuroectoderm differentiation but preserved stemness and differentiation potential.

The paper reports a draft of human tissue N-glycans of glycoRNA across 12 organs using mass spectrometry-based N-glycomics.

The paper introduces a high-throughput glycoproteomics method to quantify brain glycoproteome changes induced by gut microbiome composition.

This study identifies OGT-mediated O-GlcNAcylation of HK1 as a mechanism regulating glycolytic metabolon assembly on mitochondria, enhancing ATP production and synaptic function.

This paper identifies neolactotetraosylceramide (nLc4) as a non-invasive urinary biomarker for bladder cancer, showing higher levels in cancer tissues and urine exosomes with high specificity.

This paper introduces a computational framework for differential glycomics expression analysis, demonstrating improved robustness and reliability through specialized methods and an open-source tool.

The paper shows O-GlcNAcylation of Keratin 18 coordinates TCA cycle enzymes to promote cholangiocarcinoma progression by stabilizing K18 and altering metabolite levels.

This paper develops novel glycan probes with a tri-functional linker for generating noncovalent and covalent arrays, revealing differences in glycan binding by various proteins on different array platforms.

The paper shows that endothelial cells regulate IgG sialylation through the FcRn-mediated recycling pathway, impacting inflammatory responses.

This study shows that the physical properties of the microenvironment influence glycocalyx composition and immune surveillance in vitro using physiologically relevant models.

This paper introduces a systems-level approach to study O-GlcNAcylation by integrating AP-MS and chemoproteomics with network analysis, revealing both conserved and tissue-specific functions.

RAMZIS is an R package that improves the interpretation of glycoproteomics data by assessing the significance of glycosylation changes using similarity metrics and quality evaluation.

This study compares N-glycan processing in SARS-CoV-2 spike-proteins across variants to understand immune evasion mechanisms.

The paper identifies T829 as the primary O-GlcNAc modification site on GIGANTEA in Arabidopsis using mutational analysis and mass spectrometry.

The paper shows that an OGT variant causes reduced pluripotent stem cell self-renewal and lower stem cell marker levels in mouse embryonic stem cells.

The paper maps N-glycoproteome changes during neutrophil granulopoiesis, showing granule-specific glycosylation and remodelling driven by protein expression shifts.

The paper uses exome sequencing to identify rare variants in glycosyltransferase genes affecting transferrin and IgG glycosylation, linking them to disease-related traits.

GlyLES converts IUPAC-condensed glycan notation to SMILES using a grammar-based approach, enabling atomic-level representation and applications in molecular modeling and machine learning.

The paper characterizes N-glycosylation at synaptic vesicles and neuronal plasma membranes in mouse brain, identifying highly fucosylated N-glycans enriched in synaptic vesicles.

The paper develops a fluorescent probe to visualize O-GlcNAcylation in Drosophila embryos, showing its decline during early development and its role in neurodevelopmental gene regulation.

OxoScan-MS enables sensitive and scalable glycoproteome profiling of plasma samples, revealing differential glycosylation in severe COVID-19 patients.

The paper characterizes changes in N-glycosylation and related molecular signatures in the Parkinsonian human nigro-striatal region, linking them to endoplasmic reticulum stress and potential therapeutic implications.

This paper determines the homodimeric structure of LARGE1, a glycosyltransferase, revealing its dimerization mechanism and how disease mutations affect its function.