Jolene K. Diedrich

This study shows that mRNA vaccines can prime and mature ultrarare HIV bnAb precursor B cells in NHPs, with improved affinity using a modified immunogen.

dnoise reduces Bruker timsTOF data size with minimal impact on analysis results.

This paper introduces RIPUP, a rapid protease-based workflow for histone PTM analysis, showing improved PTM coverage and quantitative accuracy using TMT labeling and complementary proteases.

The paper identifies new ciliary proteins in the developing brain using proteomics and validates their roles in cortical development.

The paper identifies S-nitrosylation of STING as a redox modification driving aberrant cGAS-STING signaling in Alzheimer's disease, contributing to neuroinflammation and synaptic loss.

This study shows suppressing BMP signaling in astrocytes reduces molecular and functional deficits in fragile X syndrome.

This paper introduces a framework for interpreting single-cell proteomics data from patch-clamped neurons in acute brain slices, showing how retrieval quality affects proteomic outcomes.

This study uses CRISPR-Cas9 screening to identify microproteins regulating adipocyte proliferation and lipid metabolism in mice, revealing a novel mouse-specific microprotein involved in adipocyte differentiation.

Membrane-bound BG505 MD39.3 Env trimer delivered as mRNA-LNP reduced base-directed antibody responses in rabbits and macaques and elicited neutralizing antibodies, with memory B cell responses documented in macaques.

The paper examines how mutations and a deletion in the KP.3.1.1 SARS-CoV-2 spike protein affect ACE2 binding and antibody escape, revealing structural and functional insights into its evolution.

The paper presents a nanodisc platform for assembling transmembrane glycoproteins into vaccine candidates, demonstrating its utility in HIV vaccine development through structural and functional analyses.

The paper identifies Emerin as an activity-induced protein that gates neuronal plasticity by inhibiting protein synthesis and altering dendritic spine morphology.

The paper uses proximity proteomics to identify Git1 as a regulator of Smo signaling in the Hedgehog pathway, showing its role in Grk2 transport and Smo phosphorylation.

This study compares cleavable and uncleavable biotin-alkyne labels in BONCAT analysis, finding that cleavable labels improve protein identification and quantification.

This study identifies key structural and glycosylation features of EtpA, an ETEC adhesin, and shows how antibodies target different epitopes to influence immune responses and pathogen-host interactions.

This study identifies acetylation of lysine 82 in TDP-43 as a mechanism causing nuclear loss of function and cytoplasmic accumulation, linked to neurodegenerative diseases like ALS.

The paper identifies a relationship between protein structure and glycan structure, introducing a glycoimpact matrix to predict glycosylation patterns and validate it through disease variants and engineered antibodies.

The paper analyzes B cell responses to a germline-targeting immunogen in human Ig loci transgenic mice, finding diverse non-VRC01-class B cells and competitor B cells recognizing epitopes near the VRC01 epitope.

The paper uses in vivo protein footprinting to study dynamic conformational changes in the proteome across multiple tissues in Alzheimer's disease progression, finding that structural changes precede expression changes in key pathways.

This study identifies a reduction in ER chaperones in PWS β-cells, leading to impaired insulin secretion and ER stress.

The paper shows that arginine depletion during inflammation reduces nuclear ArgRS, which interacts with SRRM2, altering splicing and metabolic outcomes.

The paper identifies disorder-specific and shared changes in astrocyte protein secretion and gene expression in neurodevelopmental disorders, linking increased Igfbp2 and BMP signaling to altered neuronal development.

The paper identifies conserved spliceosomal proteins in fungi and humans that regulate splicing efficiency and suppress weak splice sites, suggesting a role in enabling intron diversity through error limitation.

This study uses cortical organoids derived from patients with 16p11.2 CNV to show altered neurodevelopmental processes, including changes in organoid size, neuronal maturation, and migration.

The paper maps O-glycosylation sites on SARS-CoV-2 spike protein produced in insect and human cells, identifying 25 sites with distinct patterns between ectodomains and RBD.

This study shows that Cul3 haploinsufficiency in mice causes social and cognitive deficits, cortical volume reduction, and cytoskeletal defects, which are rescued by RhoA inhibition.

This study identifies host proteins interacting with SARS-CoV-2 spike protein, showing that D614G mutation affects spike processing and tropism, and introduces pCtSEA to analyze cell type-specific interactions.

This study identifies tick saliva proteins injected during feeding using LC-MS/MS, finding 284 conserved proteins that may serve as vaccine targets.

The paper shows that DNA methylation persists in Cryptococcus neoformans despite the loss of a de novo methyltransferase, with methylation patterns maintained through replication and natural selection.

The paper identifies five novel genes required for RNAi-mediated transposon suppression in C. neoformans, revealing a non-Dicer RNase III and nucleolar factors involved in endo-siRNA biogenesis.