Cellular transport and secretion

The paper identifies vacuoles as a membrane source for yeast signaling endosomes, mediated by the CROP complex and regulated by TORC1 activity.

This study identifies VARP's role in forming endosomal supercomplexes with SNX27 and Retromer through direct interaction and biochemical reconstitution.

The paper identifies the client-binding site on Vps74/GOLPH3, showing it overlaps with PI4P and Arf1 binding sites and reveals a direct recruitment mechanism from the cytoplasm.

This study identifies interactions that mediate BLTP2 localization at ER-PM contacts and shows its role in controlling plasma membrane dynamics through lipid transport.

The paper investigates how Rab4 signaling via PI(3)P regulates endosomal trafficking and synaptic remodeling during development in Drosophila larvae.

The paper identifies evolutionarily conserved principles of ESCRT-III-mediated membrane remodeling using an Asgard archaeal two-subunit system, showing homology to eukaryotic ESCRT-IIIB and IIIA.

The paper shows that ESCRT-driven ILV formation and Rab5-to-Rab7 conversion are coordinated, with ESCRTs and ubiquitinated cargo regulating Rab conversion timing.

This study investigates how blocking the Sec61 translocon with mycolactone affects the production of enveloped viruses, finding that some viruses resist this inhibition early in infection.

This study identifies TMEM184B as a regulator of endolysosomal acidification in neurons by promoting V-ATPase assembly, linking it to neuronal function and disorders.

This study shows TbSec13.2 in Trypanosoma brucei is essential for COPII-mediated ER export, challenging the idea that it is restricted to the SEA/GATOR complex.

This study shows that Ca2+ binding to dEsyt modulates ER-PM MCS density in Drosophila photoreceptors, linking Ca2+ influx to signal transduction.

SuperResNET analyzes single-molecule imaging data to show that Pitstop 2 and Dynasore alter clathrin structure in distinct ways in HeLa cells.

This study shows that γ-secretase interaction with retromer enhances retromer-mediated retrograde transport of specific cargos in human cells.

This study identifies ArfR GTPases in Asgard archaea, showing they are ancestral to eukaryotic Arf family proteins and suggest early evolution of endomembrane systems.

This study shows GRASP55 regulates lysosomal enzyme trafficking by affecting HEXA sorting, M6P receptor interaction, and GNPTAB expression, leading to altered enzyme maturation and secretion.

This study identifies PKC as a regulator of peroxisome biogenesis by enhancing peroxisome-ER interactions through GSK3β inactivation, particularly during neuronal differentiation.

This study identifies TNGL-1 as a C. elegans TANGO1 family member that facilitates vitellogenin export from the ER by engaging cargo via a luminal domain.

The study directly visualizes CSC internalization and shows actomyosin and the Arp2/3 complex are involved in both exocytosis and internalization of CSCs.

The paper characterizes synaptic vesicles in iPSC-derived dopaminergic neurons, revealing distinct secretory vesicle pools with different molecular and ultrastructural features.

This study shows that acute GARP depletion causes vesicle transport defects, leading to mislocalization of Golgi proteins, O-glycosylation issues, and accumulation of vesicle-like structures.

This study identifies BORC's role in segregating synaptic vesicle and lysosomal proteins in neuronal cell bodies through motors UNC-104/KIF1A and UNC-116/KIF5, with LRK-1 and APB-3 regulating this process.

This paper proposes a lever hypothesis for Synaptotagmin-1 action in neurotransmitter release, suggesting it pulls SNARE complexes to facilitate membrane fusion.

The paper develops POTATOMap to study biosynthetic lysosomal protein trafficking in neurons, revealing domain-specific mechanisms and interactions.

This study identifies TAT-1 as a phosphatidylserine flippase required for normal endosomal function and molting in C. elegans, linking it to NEKL-2/3 and endocytic processes.

This study shows that mutations in Syt1's C2 domains impair DCV docking but increase fusion pore size and serotonin release speed.

This study determines the structural mechanism of AP-3 membrane recruitment and activation through cryo-EM, revealing conformational changes driven by Arf1 binding and cargo engagement.

The paper reports a cryo-EM structure of EFR3A bound to the PI4KA complex, revealing the molecular mechanism of PI4KA membrane recruitment and its regulation in human disease.

The paper investigates the localization of SNARE proteins in Munc18-1 deficient photoreceptors and finds that Munc18-1 prevents segregation of syntaxin-3 and SNAP-25 in presynaptic terminals.

This study identifies RRBP1 as a novel peroxisome biogenesis factor in human cells, showing it promotes peroxisome formation and stabilizes peroxisomal proteins.

The paper investigates the neofunctionalization of a Poales-specific EXO70 clade, showing HvEXO70FX12 lost exocyst complex association and may play a role in immunity.