Advanced Electron Microscopy Techniques and Applications

CryoDataBot automates the creation of high-quality cryoEM datasets for AI-driven structural biology, improving training efficiency and enabling flexible dataset customization.

This paper presents a workflow for cryo-EM structure determination of small proteins using heterogeneous ab initio reconstruction, successfully solving structures of three small proteins with resolutions below 5 Å.

The paper investigates using p-values to estimate the number of distinguishable classes in cryoEM data by testing if classifications differ from random partitions.

This paper introduces a genetically encoded FerriTag system for labeling target proteins in live cells for cryo-electron tomography, enabling precise localization with high contrast.

The paper improves the resolution of the Cryo-EM structure of E. coli type I pilus, enabling detailed analysis of water molecule interactions and subunit stabilization.

This paper introduces cryo-SXT as an intermediate technique to bridge the resolution gap in cryo-CLEM by providing 3D cellular context to cryo-ET datasets.

This paper introduces a method to validate 3D cryoEM reconstructions by separating scattering contributions into real and imaginary components to determine handedness and assess reconstruction quality.

This paper combines cryo-EM and FIB-SIMS to map sub-cellular chemical distributions in bacteria, revealing pollutant storage in phase-separated aggregates.

This paper introduces a method to reduce preferred orientation in cryo-EM samples using laser flash melting and revitrification, improving protein structure determination.

Leopard-EM accelerates in situ cryo-EM analysis by providing a modular 2DTM framework that improves ribosome detection and enables single-molecule resolution of rotation states.

This paper develops a volumetric denoising approach to improve VEM acquisition speed and downstream analysis by optimizing resolution and noise handling.

The paper validates helical symmetry parameters in EMDB by identifying and correcting inconsistencies between parameters and density maps.

Magellon provides a flexible and scalable cryo-EM platform with microservices architecture for data management, processing, and visualization.

This study uses cryogenic light microscopy to determine three conformational states of PIEZO1 in the cell membrane, revealing structural changes associated with channel opening.

This study evaluates tilt increments in cryo-ET to find the optimal balance between image quality and radiation damage, recommending 3 degrees as the best increment.

PickET localizes macromolecules in cryo-ET tomograms without expert annotations or prior structures, showing effectiveness across diverse datasets.

The paper uses cryo-EM to analyze membrane proteins, revealing unexpected high-resolution structures of ArnA and AcrB instead of the intended FtsH and YidC complex.

This paper uses cryoEM to analyze antibody-antigen interactions with HIV MPER-GT immunogens, revealing binding patterns and epitopes that inform antigen optimization.

The paper introduces Di-Gluebodies, covalently-dimerized nanobodies, to improve cryo-EM structure determination of small proteins by providing rigidity and modularity.

This paper demonstrates cryo-EM can solve protein structures directly from cell-free expression mixtures with minimal purification, reducing time and reagent use.

The paper uses cryoEELS and cryoET to study calcium distribution in mitochondria of human retinal ganglion cells under calcifying conditions, revealing mitochondrial calcification.

This paper demonstrates that a 100 keV cryo-TEM with advanced components can achieve sub-3 Å resolution structures of various proteins and viruses.

The paper introduces AIVE, an AI-assisted method for segmenting membrane boundaries in vEM data, revealing a new type of mitochondrial contact called intrusions.

The paper determines the atomic structure of the PS2 S-layer from Corynebacterium glutamicum using cryo-EM and shows its polar assembly mechanism and phylogenetic distribution.

EMSequenceFinder accurately assigns amino acid sequences to cryo-EM map backbone fragments, outperforming existing methods in resolution 3-10 Å maps.

The paper introduces an unsupervised method to analyze structural variability in cryo-EM data by deforming atomic models using normal mode analysis directly against 2D data, improving accuracy and interpretability.

This paper introduces EMERALD-ID, a method for identifying small molecules in cryo-EM data, achieving 43% exact identification and 66% close identification of common ligands.

VitriFlex is an open-source robotic platform for cryo-EM grid preparation that enables customizable and high-resolution grid production through modular design and programmable control.

The paper presents a cryo-EM conformational heterogeneity challenge to evaluate and improve methods for analyzing molecular motions in heterogeneous samples.

This paper introduces TANGO, a framework for analyzing spatial arrangements of particles in cryo-ET using point cloud descriptors, enabling rotationally invariant feature extraction and exploratory analysis of cellular structures.