Wah Chiu

This paper uses cryo-vEM to visualize and quantify ultrastructural features of two Anabaena species without chemical pre-treatment.

This study uses cryo-EM and deep learning to reveal the sequential structural changes in human TRiC during ATP-induced ring closure, showing asymmetric subunit responses and coordinated domain movements.

The paper investigates how BIM1 selectively inhibits CLC-Ka over CLC-Kb by analyzing cryo-EM structures and showing that interactions with a conserved lysine and dynamic gating-loop behavior underlie this selectivity.

This study identifies MIRO1 as a context-specific modulator of BAX activity, showing it drives distinct cell death pathways in glioma cells and Parkinsonian neurons through different mechanisms.

The paper provides a mechanistic model for the 2:1 Cl-/H+ exchange in CLC transporters using structural and computational methods.

The paper describes a Kaggle competition for RNA 3D structure prediction, showing that template-based methods can outperform deep learning approaches.

This paper evaluates blind predictions of dynamic water and ion ensembles around RNA using cryo-EM densities from CASP16, finding that molecular dynamics approaches perform best.

Structure providers for 18 CASP16 nucleic acid targets find blind predictions capture secondary structure but miss non-canonical interactions, backbone kinks, ligand sites, and intermolecular interfaces.

The paper visualizes the nucleation, condensation, and propagation of β-tubulin folding in the chaperonin TRiC, showing a hierarchical process involving nonnative structures and TRiC subunits.

The paper uses cryoEELS and cryoET to study calcium distribution in mitochondria of human retinal ganglion cells under calcifying conditions, revealing mitochondrial calcification.

The paper introduces and analyzes Q-scores to assess the reliability of atomic coordinate models fitted to 3DEM maps, showing their correlation with map resolution and model quality.

The paper uses cryogenic electron tomography to redefine herpesvirus capsid assembly intermediates inside the cell nucleus by deriving atomic models and revealing structural heterogeneity in capsid components.

Cryo-EM at 2.9-3.1 Å shows bacterial OLE, ROOL and GOLLD RNAs self-assemble without protein into a dimer, an 8-chain nanocage and a 14-chain nanocage.

Two Tetrahymena ribozyme cryo-EM maps (2.2 and 2.3 Å) reveal cross-validated ordered waters and Mg2+ ions, plus reproducible diffuse solvent density resembling molecular dynamics water networks.

This study analyzes the factors affecting resolution in cryoET-STA for MmCpn, evaluating the cost-benefit of achieving high-resolution structures.

This study shows that mitochondrial repositioning and reorientation in Chlamydomonas reinhardtii under low CO2 conditions is regulated by CIA5 and supports CO2 concentrating mechanism function.

CryoViT improves segmentation of large subcellular structures in cryoET data using vision transformers, outperforming U-Net-based methods with limited training data.

This paper introduces an unsupervised learning approach using coordinate networks to address the missing wedge problem in cryoET, improving reconstruction quality and reducing runtime.

This paper determines the 3D structures of SL5 RNA domains from six coronaviruses, revealing conserved tertiary features with implications for protein-binding and antiviral targets.

This study uses cryo-ET to analyze the structure of thin filaments in human iPSC-derived cardiomyocytes and how they change with disease-related perturbations.

This study uses cryoET and proteomics to identify mitochondrial and organelle abnormalities in HD models, showing potential for early diagnosis and therapy development.

This study uses cryo-electron tomography to reveal the molecular structures of two tubulin-based cytoskeleton structures in Toxoplasma gondii, showing their architecture and role in invasion.

This study uses cryo-electron tomography to analyze the structure of mutant huntingtin exon 1 and polyQ aggregates, revealing structural heterogeneity and a 'polyQ core' model.