Advanced Electron Microscopy Techniques and Applications

MemBrain v2 couples a generalist U-Net cryo-ET membrane segmenter, a mesh-based picker reaching 91% F1 for photosystem II from one annotated membrane, and geodesic particle statistics.

AreTomo3 and DenoisET form an automated GPU pipeline that reconstructs cryo-electron tomograms with tile-based contrast transfer function correction, screens their quality, and denoises them during acquisition.

Automated cryogenic plasma-focused ion beam milling and cryo-electron tomography of Chlamydomonas reinhardtii yield 1,829 annotated cellular tomograms and sub-nanometer in situ structures of six macromolecular complexes.

Xenon plasma FIB cryo lift-out from high-pressure-frozen mouse hippocampal slices up to 200 µm thick enables fluorescence-guided cryoET of CA1 synapses and apical dendrite cytoskeleton.

Two Tetrahymena ribozyme cryo-EM maps (2.2 and 2.3 Å) reveal cross-validated ordered waters and Mg2+ ions, plus reproducible diffuse solvent density resembling molecular dynamics water networks.

ModelAngelo combines convolutional and graph neural networks with protein language model embeddings and hidden Markov model searches to automate atomic model building and identify unknown proteins in cryo-EM maps.

RELION 2.0 adds empirical Bayesian helical reconstruction with orientation priors, filament-level half-sets and local twist/rise optimisation, yielding near-atomic maps for four filament datasets.