Sagar Khavnekar

Automated cryogenic plasma-focused ion beam milling and cryo-electron tomography of Chlamydomonas reinhardtii yield 1,829 annotated cellular tomograms and sub-nanometer in situ structures of six macromolecular complexes.

This paper demonstrates that a 100 keV cryo-TEM with advanced components can achieve sub-3 Å resolution structures of various proteins and viruses.

The paper uses cryo-electron tomography to visualize mitochondrial respiratory complexes in Chlamydomonas reinhardtii, revealing their organization into distinct membrane domains and a respirasome structure with cytochrome c binding.

TOMOMAN streamlines cryo-ET data processing by integrating tools for interoperability, metadata management, and collaborative data sharing.

The paper introduces an algorithm to improve CTF estimation in cryo-ET tilt-series data by using tilt-series alignment parameters to account for specimen geometry, enhancing resolution of subtomogram averages.

The paper introduces cryoDRGN-ET, a deep generative model for reconstructing dynamic biomolecular structures from cryo-ET subtomograms, revealing ribosome states in yeast cells.

STOPGAP is an open-source tool for subtomogram averaging in cryo-ET data to identify, localize, and classify molecular structures.

This paper introduces a fast multi-position tomographic acquisition method for subtomogram averaging, achieving sub-nanometer resolution in situ.

This paper introduces a method using metallic platinum sputtering to improve subtomogram averages, enabling sub-5 Angstrom resolution of yeast ribosomes with fewer particles.