Escherichia coli research studies

This study compares Salmonella and Shigella colonization strategies in human enteroids and colonoids, identifying distinct virulence factor modules that drive divergent infection kinetics and epithelial colonization patterns.

This paper presents cryo-EM structures of engineered Shiga toxin-based immunogens that stabilize pentamers and show interaction with neutralizing antibodies, supporting their use in a vaccine for STEC-HUS.

The paper characterizes a new Escherichia cryptic clade IV strain from an urban stream, revealing genomic features linked to environmental adaptation and ecological origin.

This study identifies PIK3C3 as essential for Shigella flexneri cell-to-cell spread by facilitating vacuole formation through PtdIns(3)P accumulation and Dynamin 2 recruitment.

This study shows antibiotic treatment affects BEV production and their ability to upregulate ICAM-1 in endothelial cells, with meropenem increasing BEV production and stimulatory capacity in E. coli strains.

This study maps CRP binding sites in EAEC to reveal its role in regulating virulence genes linked to nutritional state.

This study identifies bacteriocin genes co-occurring with antibiotic resistance and virulence factors in gut E. coli, highlighting their role in microbial dynamics and resistance dissemination.

This study shows that parenteral vaccination with recombinant EtpA glycoprotein can protect against ETEC intestinal colonization in mice, with protection dependent on vaccination schedule and adjuvant type.

This study identifies mechanoporation via the Type Three Secretion System as a mechanism by which Shigella flexneri breaches vacuolar membranes to access the cytosol.

The paper compares genomic data from blood and faecal isolates of E. coli and K. pneumoniae from neonatal patients to determine if bloodstream infections originated from the gastrointestinal tract.

This study identifies AR4 (cadA) as critical for UPEC survival during early intracellular infection by showing its induction and role in lysosome resistance.

This study examines genotype dependence of virulence-associated genes in ESBL-producing E. coli from UTI patients, finding ST131 strains show distinct virulence gene repertoires and lower sepsis rates.

The paper identifies extraintestinal pathogenic Escherichia coli, Enterococcus faecalis, and Streptococcus gallolyticus as potential causative agents of exudative cloacitis in kakapos through metatranscriptomic analysis of lesions and cloacal swabs.

The study identifies 12 accessory T3SS effectors in Citrobacter rodentium that influence infection outcomes without affecting virulence.

This study uses a CRISPRi screen to identify genes in Salmonella Typhimurium that affect multicellular aggregate formation in response to Sal4 IgA, revealing a role for Type 1 Fimbriae in evading antibody-mediated agglutination.

This study shows that the wzxE flippase is essential for E. coli survival under low pH, low temperature, and high osmotic stress by suppressing the negative effects of ECA intermediates.

This study shows Shigella flexneri uses IpaH1.4 to degrade RNF213 and prevent LPS ubiquitylation, evading host antibacterial autophagy.

This paper shows that 5-fluorocytosine inhibits biofilm formation and virulence in uropathogenic E. coli and synergizes with β-lactams to eradicate biofilm-embedded bacteria.

The paper shows that HlyF/CprA-dependent outer membrane vesicles from bacteria are more efficient at delivering cargo into host cells via a direct fusion pathway.

This paper analyzes the genetic and phenotypic role of the pAA virulence plasmid in a non-Shigatoxigenic E. coli O104:H4 outbreak strain, showing its contribution to adhesion and a functional CcdAB toxin-antitoxin system.

This paper shows that serine proteases from diarrheagenic bacteria cleave CD45 and induce T-cell apoptosis, mimicking IL-2 deprivation and contributing to bacterial virulence.

This study evaluates the protective potential of type 1 and 3 fimbriae subunits in a murine model of K. pneumoniae lung infection, showing reduced bacterial load and inflammation.

This study shows in vitro assays poorly predict the in vivo infectivity of E. coli ST131 clinical isolates in a murine UTI model.

This paper develops an OMV-based vaccine targeting intimin to reduce EHEC intestinal carriage in ruminants, showing reduced fecal shedding in a mouse model.

This study characterizes the genetic diversity and virulence factors of EPEC in southwestern Nigeria using whole-genome sequencing, identifying prevalent serotypes and potential outbreaks.

This study shows that Enteroaggregative E. coli exhibits T6SS phenotypic heterogeneity, enabling a trade-off between antibacterial activity and resistance in mixed microbial environments.

This study uses experimental evolution to show that T6SS activity is lost in EAEC when grown with immune cells, identifying mutations and a novel RfaH-dependent regulatory mechanism.

This study shows that the EPEC effector Map depletes tight junction proteins occludin and claudin-4 via cathepsin B and Rab13-mediated mechanisms, leading to barrier dysfunction.

This study characterizes the glycosylation of surface proteins in E. coli, focusing on type I fimbriae and flagella, revealing chemically resistant glycans and unexpected glycan structures.