Clostridium difficile and Clostridium perfringens research

Clostridioides difficile Toxins Unhinged: Allosterically Switchable Network Orients β-flap

The paper reveals an allosteric mechanism in Clostridioides difficile toxins using MD simulations and mutagenesis, showing a switchable interaction network that drives conformational changes.

Inhibition of EGFR/ErbB does not protect against C. difficile toxin B

This study investigates whether inhibiting EGFR/ErbB receptors protects against C. difficile toxin B, finding no protective effect.

Genome sequence and characterization of a hypervirulent BI/NAP1/027 Clostridioides difficile (CDC20121308)

The paper characterizes the genome of a hypervirulent C. difficile strain, identifying genes associated with pathogenesis and antibiotic resistance.

Approaching pathogenic Clostridia from a One Health perspective

The paper discusses the use of genomic resources for spore-forming pathogens like Clostridia to improve outbreak detection, disease prevention, and understanding of their persistence and virulence across environments.

Systems-ecology designed bacterial consortium protects from severe Clostridioides difficile infection

The paper develops a systems biology pipeline to design bacterial consortia that protect against severe Clostridioides difficile infection by targeting amino acid cross-feeding.

A network of small RNAs regulates sporulation initiation in C. difficile

The paper identifies a network of small RNAs that regulate sporulation in C. difficile by modulating translation of the master sporulation regulator Spo0A.

Personalized Clostridioides difficile engraftment risk prediction and probiotic therapy assessment in the human gut.

The paper uses microbial community-scale metabolic models to predict C. difficile engraftment risk and assess probiotic therapy efficacy in the human gut.

Molecular characters and phylogenetic analysis of Clostridium perfringens from different regions in China, from 2013 to 2021

The paper analyzes genetic diversity and antibiotic resistance of Clostridium perfringens isolates from humans, animals, and food in China using MLST, cgMLST, and cgSNP, revealing high genetic diversity and potential transmission events.

Microbial interactions impact the growth response of Clostridioides difficile to antibiotics

This study identifies microbial interactions affecting C. difficile's antibiotic response using human gut communities and transcriptional profiling.

K-mer based prediction of Clostridioides difficile relatedness and ribotypes

This paper uses MinHash to rapidly approximate genomic distances and predict ribotypes in Clostridioides difficile genomes, reducing computational demands for transmission analysis.

Predictive regulatory and metabolic network models for systems analysis of Clostridioides difficile

The paper develops regulatory, metabolic, and integrated models for C. difficile to understand its colonization mechanisms and interactions with the gut microbiota.

Species richness determines C. difficile invasion outcome in synthetic human gut communities

This study shows that species richness in synthetic human gut communities determines the outcome of C. difficile invasion, with mechanisms like acidification and resource competition playing key roles.

Performance of Core Genome Multilocus Sequence Typing Compared to Capillary-Electrophoresis PCR Ribotyping and SNP Analysis of Clostridioides difficile

The paper compares core genome MLST (cgMLST) with CE-PCR ribotyping and SNP analysis for C. difficile typing, finding cgMLST offers better discrimination but may require adjusted thresholds for outbreak detection.

Phylogenomic analysis of Clostridiodes difficile ribotype 106 strains reveals novel genetic islands and emergent phenotypes

The paper identifies novel genetic islands in Clostridiodes difficile RT106 strains and links them to emergent phenotypes contributing to their dominance.

Spo0A suppresses sin locus expression in Clostridioides difficile

This study shows that Spo0A regulates the sin locus in C. difficile, controlling sporulation, toxin production, and motility by binding to its upstream region.

Identification of the C. sordellii lethal toxin receptor elucidates principles of receptor specificity in clostridial toxins

The paper identifies SEMA6A and SEMA6B as receptors for C. sordellii lethal toxin and shows how mutations can switch receptor specificity between related toxins.

Cell-specific targeting by Clostridium perfringens β-toxin unraveled: the role of CD31 as the toxin receptor.

This paper identifies CD31 as the receptor for Clostridium perfringens β-toxin, explaining its cell type specificity and toxicity.

C. difficile-associated antibiotics prime the host for infection by a microbiome-independent mechanism.

This study shows that certain antibiotics increase CDAD risk through host-driven changes, not just microbiome disruption, by impairing mucosal barriers and immune function in human gut models.

Epigenomic and functional characterization of a core DNA methyltransferase in the human pathogen Clostridium difficile

This study characterizes the epigenome of C. difficile and identifies a conserved DNA methyltransferase that impacts sporulation and other biological processes.

A global to local genomics analysis of Clostridioides difficile ST1/RT027 identifies cryptic transmission events in a northern Arizona healthcare network

This study uses whole genome sequencing to analyze C. difficile isolates and identifies multiple introductions and transmission events in a healthcare network, highlighting genetic variability and antimicrobial resistance.

Long-term taxonomic and functional divergence from donor bacterial strains following fecal microbiota transplantation in immunocompromised patients

This study examines how bacterial composition and function change over a year after fecal microbiota transplantation in immunocompromised patients, finding limited long-term donor strain persistence and complex functional shifts.

Gut Microbiota Composition Related With Clostridium difficile-Positive Diarrhea And C. Difficile Type (A+B+, A-B+, And A-B-) In ICU Hospitalized Patients

The paper compares gut microbiota composition in ICU patients with Clostridium difficile-positive diarrhea and different C. difficile types using 16S rDNA and metagenome sequencing.