Click Chemistry and Applications

This paper develops a photoclickable labeling system for precise spatiotemporal protein labeling on living cells using visible light activation.

This paper develops vCATCH to map covalent drug targets across the entire mouse body, revealing new tissue and cell type engagements for afatinib and ibrutinib.

INTERACT identifies and quantifies active enzymes in host-pathogen interactions during infection without genetic manipulation.

This paper designs aromatic cation salts that enable photo-induced protein labelling and imaging using longer wavelengths of light, achieving robust reactivity in live cells.

This paper develops second-generation acyl silane photoaffinity probes for cellular chemoproteomic profiling, showing improved labeling properties compared to existing diazirine-based probes.

The paper identifies electrophilic compounds that covalently bind RNA and uses this insight to design an RNA-specific covalent binder for myotonic dystrophy type 1.

This paper develops an in vivo method to covalently link peptide drugs to native IgGs, demonstrating sustained weight loss and glucose management with GLP-1.

OpenEMMU improves EdU staining efficiency, brightness, and multiplexing for DNA replication and cell cycle analysis using affordable, open-source reagents.

This paper introduces a multicopy MetRS* mouse line to enhance cell type-specific in vivo protein labeling, enabling sensitive detection of newly synthesized proteins in sparse cell populations.

The paper identifies protein ligands for monoacylglycerol lipids using chemical proteomics in mouse brain and cell lysates, revealing Hippocalcin as a novel ligand involved in calcium sensing.

This study develops a highly reactive and stable tetrazine amino acid for site-specific protein labeling, demonstrating improved encoding and ligation efficiency.

The paper develops a method to modify receptor proteins in the living mouse brain with fluorescent probes using ligand-directed and click chemistry, enabling high-resolution mapping of matrix metalloproteinase-9 activity near AMPARs.

This paper introduces two novel methods to incorporate DBCO into S. aureus peptidoglycan for SPAAC-mediated cargo labeling, showing improved stability and reduced toxicity compared to existing approaches.

This paper introduces a method to site-specifically modify growth factors with azido tags using glycosylation for biomaterial conjugation, demonstrating enhanced angiogenic responses in hydrogels.

This study introduces MA-diyne, a click chemistry probe for detecting lysine malonylation in proteins, revealing its role in osteoarthritis by identifying targets and showing Sirtuin 5 regulation.

The paper uses stereoprobes to identify covalent ligands for proteins, revealing TLCD1 as a lipid acyltransferase inhibited by OTP stereoprobes.

The paper introduces DNADetect™ probes as an EdU alternative for DNA labeling in mammalian cells, bypassing thymidine kinase dependency and demonstrating efficient DNA incorporation.

The paper develops a chitin labeling method to study hyphal growth and homeostasis in Candida species, revealing mechanisms of morphological switching and potential antifungal targets.

The paper introduces photo-click adapters for optical and chemical cell surface tagging, demonstrating applications in zebrafish embryo imaging and combinatorial cell labeling.

This study uses genetic code expansion to site-specifically lactylate proteins in living cells, revealing lactylation's impact on enzyme activity and cellular function.

This study introduces a pH-controlled regioselective rapid ene-type reaction (RRER) for selectively modifying histone H3Q5 serotonylation and measuring its levels in cells and tissues.

This paper develops bioorthogonal cyclopropenones to crosslink RNA and study its native structures in cells.

The paper introduces photo-stereoprobes for expanding the ligandable proteome and demonstrates their use in discovering bioactive small molecules.

The paper develops covalent probes to target Legionella pneumophila effector enzymes, demonstrating their effectiveness in capturing DupA through in vitro testing and structural analysis.

The paper develops a chemical proteomic method to globally profile serotonylation in cancer cells, identifying over 1,000 serotonylated proteins and their modification sites.

This paper introduces fluorogenic probes to detect protein-protein interactions in both fixed and living cells, using dual-substrate recognition and antibody or genetic code expansion for detection.

The paper introduces CG-SLENP, a chemical genetics method to selectively label existing and newly synthesized proteins, showing differential modulation of these pools by a small molecule ligand.

DrugMap quantitatively analyzes cysteine ligandability across 416 cancer cell lines, revealing redox state, conformational changes, and mutations as drivers of variability, and identifies covalent inhibitors targeting NFκB1 and SOX10.

The paper improves the synthesis of C15AlkOPP and uses it to label and analyze prenylated proteins in motor neurons, astrocytes, and their stem cell progenitors, identifying 54, 50, and 84 prenylated proteins respectively.

This paper introduces a bioorthogonal labeling method to fluorescently visualize gas vesicles in cells, enabling analysis of their size and distribution.