Zebrafish Biomedical Research Applications

This study evaluates the effects of electrical stunning on stress response and tissue integrity in larval zebrafish, finding no significant stress response and preserved brain tissue.

This study creates a zebrafish Runx1 lineage tracing tool to track hematopoietic development and adult blood cell lineages.

This study uses zebrafish embryos to screen chemicals for developmental toxicity and identifies mechanisms, showing calcium homeostasis is involved in purpurin toxicity.

Two-photon optical backfills of pan-neuronal photoactivatable GFP in larval zebrafish yield an estimated 1,300 spinal-projecting neurons per larva across hindbrain, midbrain and diencephalon, with tentative homologies to mammalian nuclei.

This paper develops a computational method to map full 3C-3D blood flow in the beating zebrafish heart using SPIM and microPIV techniques.

This study evaluates how test order affects behavioral outcomes in zebrafish using a three-assay battery, finding modest, domain-specific effects without altering overall phenotypic structure.

The paper shows that blastema cells in zebrafish fin regeneration have intrinsic migratory capacity but osteoblasts do not migrate off the bone unless multiple injuries are present.

This paper describes a zebrafish model lacking catecholamines, showing viability with normal anatomy but impaired physiological and behavioral functions.

This study develops fully automated methods using FIJI/ImageJ to compute body morphometric measures from microCT scans of adult zebrafish, improving throughput and reducing variability.

The paper reveals that neural innervation transforms the zebrafish yolk sac from a nutrient reservoir into a functional hub coordinating neural, vascular, and metabolic activities during early development.

The paper introduces ImPaqT, a Golden Gate-based toolkit for zebrafish transgenesis, enabling rapid and flexible transgene construction and immune reporter line generation.

The paper shows that mucus cell and ionocyte precursors migrate between epithelial layers in zebrafish epidermis to achieve their scattered distribution.

This study shows that disrupting VRAC function in zebrafish leads to depression- and anxiety-like behaviors and altered gene expression, while VRAC activation improves these phenotypes.

This paper develops threshold-free deep learning models for detecting swim bouts in larval zebrafish, showing improved precision over traditional methods and revealing behavioral patterns across genetic backgrounds.

This paper introduces a high-precision pneumatic device for inducing traumatic brain injury in larval zebrafish, showing improved reproducibility and consistent injury severity measurements.

This study shows that adult zebrafish benefit from group foraging through coordination and emotional buffering, while larvae do not, highlighting developmental differences in social behavior.

The paper uses intravital imaging in zebrafish to show age-dependent changes in collective T cell migration patterns in fins, linking tissue structure and immune function.

The paper investigates the role of intracellular pH dynamics in zebrafish larval tail regeneration, finding that pH changes are critical for cell proliferation and tissue repair.

This study develops a chemogenetic ablation model in zebrafish to study ENS neuron regeneration, showing complete restoration of most neuron subtypes but partial recovery of serotonergic neurons.

This study identifies the developmental origins of zebrafish schooling behavior and shows it is a collective strategy for adapting to environmental geometry.

This study shows that larval zebrafish adjust their group behavior based on past experiences with population density, using a state-space model to predict these changes.

This study shows that testing juvenile zebrafish in groups rather than isolation reduces stress, improves behavioral stability, and enhances detection of nicotine reward.

This study uses larval zebrafish to assess the behavioral and neural effects of fentanyl and other synthetic opioids, identifying potential mechanisms and validating a scalable model for hazard assessment.

This study identifies three parallel computational pathways underlying phototaxis behavior in zebrafish, showing larvae use spatio-temporal cues while juveniles rely on ambient luminance.

The paper evaluates 4-PBA and its analogs for treating STXBP1 disorders using zebrafish models and finds no rescue of movement or seizure phenotypes.

This study develops an in vivo zebrafish screening assay to identify drugs that suppress convulsant-induced neuronal hyperexcitability, finding that Kv7 channel activators are consistently effective.

This study performs a systematic review and meta-analysis of pharmacological and stress-related interventions on social behavior in adult zebrafish, finding inconsistent effects and methodological limitations.

This study shows serotonin via 5-HT2A receptors promotes ionocyte proliferation in zebrafish larvae under osmotic stress.

This paper optimizes an optomotor response protocol for juvenile and adult zebrafish to measure visual acuity and neural function.

The paper introduces BOOPTHAT, a low-cost system for spatiotemporal activation of heat-shock transgenes in zebrafish embryos using 3D printed components and optical fiber probes.