Zebrafish Biomedical Research Applications

This paper develops a non-invasive light-inducible split-Cre system for precise genome engineering in zebrafish.

This study compares three diets' effects on growth and spinal cord regeneration in larval zebrafish, finding significant differences in length and regeneration rates between live feed and commercial feeds.

OptoPi is an open-source platform for small animal behaviour analysis with custom illumination and optogenetic stimulation, demonstrating tracking of zebrafish and Drosophila larvae.

The paper identifies otpb as a Lef1-dependent gene required for crhbp expression in zebrafish hypothalamic neurons, linking Lef1-mediated neurogenesis to stress response inhibition.

The paper identifies IGFR/mTOR signaling as a driver of increased translation during zebrafish fin regeneration, accelerating outgrowth through coordinated cell behavior.

This study uses single-cell RNA sequencing to identify molecular differences between motoneuron types in larval zebrafish that contribute to their roles in swimming behavior.

The paper shows that modulating Embryonic Neuronal Excitability (ENE) in zebrafish embryos affects dopaminergic neuron numbers and larval locomotion, with implications for neurodevelopmental disorders.

The paper examines the effects of caldesmon mutations in zebrafish embryos, finding mild developmental impacts and normal gross development, suggesting caldesmon may be a viable cancer therapy target.

This study identifies the role of progenitor-derived glia in zebrafish spinal cord regeneration through genetic and molecular analyses.

This study investigates the role of 5-HT2C receptors in zebrafish social behavior using pharmacological manipulations and behavioral tests.

This study shows that early-life stress in zebrafish affects anxiety-related behaviors and these effects are transgenerational across three generations.

This study shows that arnt1 and arnt2 genes are required alongside npas4l for hemato-vascular specification in zebrafish embryos, with each gene necessary but not sufficient alone.

This study examines the effects of quinpirole, a dopamine D2/D3 receptor agonist, on behavior and neurotransmitter levels in adult zebrafish, finding altered locomotion, anxiety, memory, and neurotransmitter profiles.

The paper introduces an efficient CRISPR-Cas9-based knock-in method in zebrafish for lineage tracing and generates reporter lines for specific cell types.

The paper describes how ZFIN annotates phenotype, expression, and disease model data in zebrafish research across different experimental designs.

The paper identifies three distinct neural pathways in larval zebrafish that mediate habituation to dark looming stimuli, with one pathway specifically inhibiting escape responses.

This study evaluates a semi-automated tool to quantify craniofacial variations in zebrafish models of human skeletal diseases, finding phenotypic differences for four genes and parallels to human symptoms for two.

The paper shows that actomyosin contractility in olfactory placode neurons causes the skin epithelium to rupture, forming the nostril in zebrafish.

The paper generates a transparent killifish line by inactivating three pigmentation genes, enabling in vivo studies of aging and behavior.

This study investigates the behavioral effects of rbfox1 deficiency in zebrafish, finding multiple behavioral changes that resemble those in Rbfox1-deficient mice and psychiatric patients.

The paper creates a single-cell gene expression atlas of the larval zebrafish brain using HCR, identifies a novel cerebellar cell type, and integrates gene expression with functional activity maps.

This study identifies Duox as a key NADPH oxidase for ROS production and fin regeneration in adult zebrafish, with secondary roles for Nox1-4 and a circadian rhythm in ROS levels.

The paper characterizes a zebrafish gabra1 loss of function allele, showing decreased mRNA expression and seizure-like phenotypes, supporting previous findings on GABRA1's role in nervous system development.

The paper introduces SAMPL, a high-throughput system for measuring posture and locomotion in small animals, demonstrating its use in zebrafish and showing genetic and developmental effects on movement.

The paper introduces next-generation transgenesis tools for zebrafish and other species, including vectors with fluorescent markers and promoters for specific tissue expression.

This review meta-analyzes 38 studies on unpredictable chronic stress in zebrafish, finding increased anxiety/fear and cortisol, decreased locomotor function, but no significant effect on social behavior, while highlighting methodological issues.

This study identifies Zbtb14 as a regulator of monocyte and macrophage development by inhibiting PU.1 expression in zebrafish, with implications for myeloid disorders.

The paper introduces DAMAKER, a 3D tool for tracking tissue growth and neuronal differentiation in zebrafish hindbrain over time using in vivo imaging and segmentation.

This study identifies a cerebellar-prepontine circuit that mediates tonic immobility in larval zebrafish in response to inescapable threats.

This study shows that Pcdh18a in stromal cell progenitors is essential for hematopoietic niche formation in zebrafish.