Zebrafish Biomedical Research Applications

This study identifies a neural network connecting the preoptic area and habenula in larval zebrafish that supports thermoregulation through movement and spatial navigation.

This study uses zebrafish to examine the effects of CSNK2A1 mutations on morphology, showing a link between genetic changes and phenotypes, with partial rescue by wild-type mRNA.

The paper investigates cell lineage relationships in zebrafish fin regeneration using single-cell RNA sequencing and lineage tracing, revealing compartmentalized osteoblast and blastema contributions to bone formation.

The paper identifies novel tagln-positive mural cell populations in zebrafish embryos using transgenic markers and reveals their developmental contributions.

This study identifies BMP, FGF, Notch, and Shh signaling pathways as critical for gill regeneration in zebrafish following filament resection.

This paper develops a high-throughput method to count EGFP-expressing neutrophils in zebrafish larvae using a parallelized microscope and machine learning, validated against manual counts.

This study shows that warming reduces routine swimming in larval zebrafish and alters molecular responses to novel odours, with energy trade-offs in gene expression.

This study identifies Alk1/Endoglin signaling as critical for restricting vein endothelial cell sizes and preventing arteriovenous malformations in zebrafish embryos.

The paper investigates the distinct roles of core autophagy-related genes in zebrafish definitive hematopoiesis, revealing gene-specific and synergistic effects on hematopoietic stem cells and myeloid cell types.

The paper introduces an all-optical technique for brain-wide calcium imaging and optogenetic stimulation in larval zebrafish, showing it can trigger behavior and neural activity changes.

This paper introduces KimmelNet, a deep learning model that predicts zebrafish embryo age from 2D brightfield images and detects developmental delay between populations.

The paper develops a robust strategy for generating knock-in reporter animals using minicircle technology and an ectopic promoter, improving efficiency and reducing unintended genomic effects.

The paper introduces a low-cost, open-source system for tracking larval zebrafish behavior during functional microscopy.

The paper evaluates the effects of 162 FDA-approved drugs on zebrafish embryogenesis and immune response to SARS-CoV-2 spike protein, identifying potential developmental side effects.

The paper maps neuromodulatory systems in zebrafish telencephalon using transcriptomics, revealing cell type-specific GPCRs and neuropeptide combinations.

This study shows adgrl3.1-deficient zebrafish exhibit externalizing behaviors rescued by atomoxetine, and identifies differentially expressed genes linked to externalizing disorders.

The paper identifies evolutionary conserved and specialized pathways in the zebrafish cortico-basal ganglia circuit using transgenic tools and single-cell RNA sequencing.

This study shows adult zebrafish can regenerate jaw joint ligaments without scarring through dedifferentiation of ligamentocytes and ECM remodeling, with legumain playing a key role.

The paper identifies genetic loci associated with motivated behavior in zebrafish and links them to psychiatric risk genes in humans.

This study identifies the mesencephalic locomotor region's role in recruiting V2a reticulospinal neurons to control forward locomotion in zebrafish.

The paper shows that endothelial cells from the dermomyotome contribute to the dorsal aorta and support hematopoietic stem cell emergence in zebrafish.

The paper investigates the molecular mechanisms governing the emergence of pre-hematopoietic stem cells from the hemogenic endothelium in zebrafish embryos, showing that apicobasal polarity and junctional recycling play key roles in their morphodynamic complexity.

This study identifies acta1b as the most suitable zebrafish gene for modelling human actin cardiomyopathies due to its expression and CRISPR-induced cardiac phenotypes.

This study identifies a single base pair substitution in the syt7a gene in zebrafish linked to an acoustic startle threshold phenotype but shows it does not cause the observed behavioral changes.

This study investigates how varying acute stressor intensity affects social approach and maintenance in adult zebrafish, showing intensity-dependent reductions in both behaviors with social maintenance affected at lower stress levels.

The paper describes a 3D-printed device to measure the optokinetic response in zebrafish before and after optic nerve injury.

The paper develops genetic tools for the mangrove killifish, improving embryo yield and enabling targeted gene knockout studies.

The paper introduces Kita as a target for CRISPR/Cas9 screens in zebrafish larvae to improve the reliability and efficiency of genetic screens for complex traits.

This paper develops a zebrafish knock-in reporter line for the Foxo1a transcription factor to enable in vivo imaging of its expression and localization.

The paper identifies a macrophage subpopulation that mediates airineme-mediated signaling through MMP9-dependent mechanisms in zebrafish.