Zebrafish Biomedical Research Applications

This paper investigates the role of f3a in zebrafish development, finding that its knockdown causes defective angiogenesis and mild bleeding.

This study compares swimming patterns and neuronal circuits in two closely related fish species to understand how environmental factors drive evolutionary divergence in locomotion.

The paper shows V3 interneurons are active during fictive swimming in larval zebrafish and contribute to locomotor amplitude but not frequency.

The paper identifies a novel site of late vasculogenesis in zebrafish embryos that contributes to functional blood vessels after circulation is established.

The paper uses calcium imaging to study brain-wide visual habituation networks in zebrafish, finding altered network dynamics in fmr1 mutants linked to slower habituation.

The paper describes ZFIN, a central repository for zebrafish genetic and genomic data, emphasizing its role in curating, integrating, and making data FAIR for research.

This paper introduces a method for long-term imaging of live adult zebrafish using a 3D printed chamber, enabling nearly day-long observation of neutrophil recruitment in transgenic fish.

The paper documents the stepwise development of the zebrafish pectoral fin vascular network using high-resolution imaging, revealing a stereotyped process involving initial vascular loops and three distinct plexuses.

This study shows that the neuropeptide Pth2 in zebrafish affects anxiety and social behavior, with altered responses to stimuli and changes in social preference during development.

The paper uses voltage imaging to identify spinal circuits in zebrafish that modulate locomotor adaptation by characterizing a novel sub-population of V3 neurons.

The paper identifies genes involved in human bone disease by analyzing transcriptomic changes during zebrafish scale regeneration.

This study uses scRNA-seq to analyze MET and EMT programs in zebrafish fin regeneration, showing co-expression of EMT markers in dedifferentiating and differentiating osteoblastic cells.

The paper develops a zebrafish assay to study alcohol preference, finding a biphasic response where low alcohol concentrations are attractive and high concentrations are avoided.

This paper presents a 3D image analysis pipeline for quantifying zebrafish brain vasculature, showing it is robust and provides novel insights into vascular biology.

This study investigates the roles of Csf1rs and their ligands in zebrafish myelopoiesis, revealing distinct functions in macrophage and granulocyte differentiation.

The paper shows that WASp regulates endothelial cell migration directionality in zebrafish, leading to proper vascular patterning and preventing vascular malformations.

The paper identifies Pizotifen as an anti-metastasis drug by screening chemicals that disrupt zebrafish gastrulation and show it suppresses EMT-mediated metastasis through HTR2C inhibition.

This study develops a zebrafish model for CDKL5 Deficiency Disorder to investigate disease mechanisms and screen for therapies.

The paper introduces ZAF, an open-source automated feeding system for aquatic facilities, which provides standardized food distribution and is adaptable to various laboratory environments.

The paper introduces AZBA, a 3D adult zebrafish brain atlas created using tissue clearing and imaging techniques, providing a detailed segmentation of over 200 neuroanatomical regions.

The paper studies how larval zebrafish transform visual inputs from neighbors into movement decisions during schooling behavior, finding that young larvae use averaging and winner-take-all strategies, while mature fish incorporate attraction to low retinal clutter.

Q-STARZ assesses spatial and temporal activity of wild-type and mutant human CRE alleles in zebrafish using live imaging and transgenic reporters.

This study shows that hypomyelination in zebrafish larvae disrupts axonal conduction and behavior, linking myelination to neural circuit function.

The paper investigates the role of 5-HT2C receptor agonists and antagonists in modulating zebrafish behavioral responses to different types of threat.

This study examines the effects of MK-801 and amphetamine on zebrafish behavior and oxidative stress, finding MK-801 disrupts social interaction and locomotion, while amphetamine increases lipid peroxidation.

The paper describes a method to generate endogenously tagged zebrafish knock-in lines by inserting fluorescent markers into non-coding regions of genes.

This study builds and simulates spinal circuit models in developing zebrafish to understand how locomotor movements transition from coiling to swimming.

This study identifies and characterizes maternal-effect genes in zebrafish using CRISPR-Cas9 to generate maternal crispants, revealing two new genes and validating known ones.