Advanced NMR Techniques and Applications

This paper introduces a method to control pH in NMR samples using light-activated photoacids, enabling non-invasive and precise pH modulation for studying protonation states in proteins.

This study determines the structure of Mycobacterium tuberculosis CrgA, revealing its dimeric form and interactions with divisome proteins essential for cell division.

This study investigates E. coli metabolism on pyruvate as the sole carbon source for NMR studies, revealing metabolic inefficiencies that impact isotopic labeling.

The paper develops a cost-effective method for producing isotopically-labeled proteins in E. coli by optimizing nutrient use and reducing 13C-D-glucose consumption.

The paper provides a rigorous theoretical interpretation of the transverse spin relaxation rate {Gamma} 2, showing it is proportional to norm and offers an explicit formula for calculating norm without adjustable parameters.

This paper introduces a Hadamard encoding method for multi-dimensional solid-state NMR spectral editing of protein sidechains, enabling unambiguous detection of signals from basic amino acids and their bound water molecules.

This study uses DNP-enhanced ssNMR to rapidly characterize native bacterial biofilms without labeling, identifying polysaccharides, proteins, and eDNA.

This paper develops a deep neural network, FID-Net-2, combined with custom NMR experiments to accurately reconstruct high-resolution aromatic 1H-13C correlation spectra from uniformly 13C-labeled protein samples.

This study evaluates AsymPolPOK's stability and performance as a polarization agent in mammalian cells, showing its effectiveness with electroporation but limitations due to rapid reduction in the cellular environment.

This study uses high-resolution 2D solid-state NMR to analyze native nontuberculous mycobacteria, identifying cell-wall chemical sites and structural differences between strains.

This paper develops an NMR method to measure dynamics in the fd bacteriophage coat protein, revealing helical motion and flexible regions.

This study uses solid-state NMR to investigate the conformational dynamics and interactions of high-molecular weight hyaluronic acid in a hydrated environment.

The paper identifies fluoride contamination from glass NMR tubes and proposes a simple solution to mitigate it in biomolecular applications.

This paper uses deep neural networks to process methyl-TROSY NMR spectra of non-deuterated proteins, achieving results comparable to deuterated samples.

This paper improves the accuracy of exchange parameters in CEST experiments by using K instead of kex to guide B1 field selection, demonstrating better results in two FF domain mutants.

The paper determines the dimeric structure of VanX using NMR and identifies a monomeric form for inhibitor screening.

This paper demonstrates a method to overcome the re-protonation bottleneck in NMR by using deuterated cell lysates, enabling high-resolution amide and Hα MAS NMR with improved sensitivity.

The paper demonstrates that deuteration via deuterated amino acids improves spectral resolution for large proteins in solid-state NMR by avoiding proton back-exchange issues.

This study uses high-resolution 1D and 2D MAS ssNMR to characterize native Pseudomonas fluorescens biofilms without isotope labeling, identifying chemical sites and distinguishing protein and polysaccharide components.

The paper introduces SpecDB, a relational database for archiving NMR FID data and metadata, to support machine learning in biomolecular NMR analysis.