Plant biochemistry and biosynthesis

The paper identifies Moraceae-specific P450 enzymes involved in furanocoumarin biosynthesis by analyzing evolutionary patterns and functional divergence in Ficus carica.

The paper reviews putative reaction pathways for the biosynthesis of sesquiterpene coumarins derived from 7-farnesyloxycoumarins, highlighting their evolutionary link to triterpene biosynthesis.

The paper deconstructs and reconstructs diterpene structures to categorize patterns and model synthesis mechanisms, revealing core skeletons and predicting compound diversity.

This paper demonstrates the production and functionalization of heterologous sesquiterpenoids in the plastid of Chlamydomonas reinhardtii using STPs and soluble CYPs, achieving up to 80% functionalization and 250-2500 µg L-1 yields.

This study identifies and characterizes three new CYPs in T. litoralis that catalyze specific oxidations of four MIA skeletons, expanding understanding of MIA biosynthesis.

The paper identifies cassava accessions with enhanced β-carotene content and examines its relationship with starch, lipid, and protein biosynthesis pathways.

This study models how circadian rhythms affect terpenoid precursor biosynthesis in plants, showing that combined regulation maintains stable concentrations despite daylight variations.

The paper identifies the biosynthetic origin of the methoxy group in quinine and related Cinchona alkaloids, showing it is introduced onto tryptamine and demonstrating the pathway in planta.

The paper constructs a cell-type-aware gene regulatory network for MIA biosynthesis in C. roseus using single-cell multi-omics data, identifying a novel idioblast-specific TF that regulates late-stage biosynthetic genes.

This study identifies WRI1 orthologues in rubber-producing plants that enhance natural rubber quality and production through regulation of lipid metabolism and biosynthesis pathways.

This study identifies two phylogenetically distant enzymes, CfADH1 and CfAKR2b, localized in cytosol and plastids, that drive citral biosynthesis in lemongrass.

This paper identifies and characterizes pachysiphine synthase genes in Tabernaemontana elegans using genome sequencing and computational analysis, enabling engineered yeast production of MIAs.

The paper solves the crystal structure of caryolan-1-ol synthase with and without a substrate analog, revealing structural changes that suggest a mechanism for anti-Markovnikov cyclization.

This study shows that substrate channeling in PaFS occurs without a flexible linker or covalent domain linkage, and that cyclase domains associate with the prenyltransferase octamer for optimal channeling.

The paper identifies two CYP450 enzymes in Tabernanthe iboga responsible for oxidative modifications of tabersonine scaffolds using a streamlined screening strategy in Nicotiana benthamiana.

This paper identifies and functionally characterizes CYP76BK1 orthologs across Lamiaceae subfamilies, showing their role in furanoclerodane biosynthesis and evolutionary conservation.

The paper presents a chromosome-level genome of Salvia divinorum, identifying gene clusters involved in salvinorin A biosynthesis.

This study identifies and characterizes a new gammaTMT-like enzyme from Tabernaemontana elegans, showing parallel evolution of methyltransferases leading to perivine N-methylation in two plant species.

The paper develops Triterpene RDF, a manually curated database of plant enzymes and transcription factors involved in triterpene biosynthesis, to improve functional annotation of genomes and transcriptomes.

This study examines how pipecolic acid treatment affects terpenoid composition in different tissues of tansy plants, revealing tissue-specific responses and metabolic differentiation.

The paper identifies Hyper cells in St. John's wort where hyperforin biosynthesis occurs de novo and characterizes four prenyltransferases involved in its synthesis.

This study compares metabolomes of wild and cultivated T. cuspidata, finding wild species have higher metabolic diversity and environmental adaptability due to genetic resources.

Three CYP716 enzymes in Nicotiana attenuata catalyze distinct steps in triterpene biosynthesis, influencing growth and development through ABA and stress responses.

This study identifies biosynthesis pathways and regulatory mechanisms of furanocoumarins in Angelica dahurica using multi-omics data and experimental validation.

The paper identifies one-step anthranilate methyltransferases in grapes that synthesize methyl anthranilate, revealing distinct active site residues compared to maize and citrus enzymes.

The paper identifies biosynthetic genes and pathways for phenylphenalenones and linear diarylheptanoid derivatives in Musella lasiocarpa using transcriptomics and biochemical assays.

The paper identifies four enzymes that control stereochemistry at carbon 5 of cardenolides in Erysimum, showing how natural variation in one enzyme explains structural diversity in these defensive compounds.

This study performs transcriptome analysis of Tylophora indica to identify genes involved in secondary metabolite biosynthesis and genetic diversity.

This study shows that the light-regulated transcription factor SlHY5 controls steroidal glycoalkaloid biosynthesis and fungal tolerance in tomato.

This paper develops a sustainable method to produce agarwood-like sesquiterpenoids using synthetic biology in algae, avoiding the need for harvesting endangered trees.