ATP Synthase and ATPases Research

The paper determines the Cryo-EM structure of the Arabidopsis thaliana V-type ATPase, revealing its catalytic cycle and organelle-specific interactions with TLDc proteins.

This paper models proton transport in mitochondria, showing cardiolipin creates a nanoscale barrier that confines protons, enabling efficient lateral transport for ATP synthesis.

This study identifies the keto form of oxaloacetate as the substrate for Vibrio cholerae decarboxylase and elucidates the proton transport mechanism during catalysis.

The paper identifies a planar dimeric form of bovine ATP synthase with minimal membrane bending, contrasting with canonical dimers that induce significant curvature.

The paper develops a minimal chemo-mechanical Markov model for F1-ATPase rotary catalysis, showing that four beta-subunit conformations are required and reconciling the bi-site versus tri-site controversy.

This paper shows that bedaquiline inhibits the ATP synthase c-subunit leak channel and prevents glutamate-induced neuronal death in primary hippocampal neurons.

The paper identifies proteolytic processing of the F1-ATPase subunit α in Trypanosoma brucei, showing that cleavage occurs in the cytosol before mitochondrial import.

The paper investigates altered ATP synthase dynamics in senescent hiPS-cardiomyocytes, linking structural changes in mitochondria to reduced enzyme activity and impaired cardiac function.

This study investigates the inhibitory effects of bisphosphonates on Thermotoga maritima mPPase, revealing conformational asymmetry and symmetry-breaking during catalysis.

The paper characterizes the functional and structural properties of an ancestral F1-ATPase, revealing it was originally a 6-stepper that diversified into 3-, 6-, and 9-step forms.

This study uses hybrid simulations to show that side chain flexibility and angular mismatch enhance Fo motor rotation efficiency, with conserved structural features across species.

The paper investigates the γ-subunit rotation mechanism in F1-ATPase using cryo-EM structures and molecular dynamics simulations, proposing the distortion-push mechanism.

This study engineered ATP synthase to increase the H+/ATP ratio beyond natural limits, enabling ATP synthesis at lower proton motive force.

This study investigates the role of If1 inhibitory peptides in Saccharomyces cerevisiae, showing they prevent ATP hydrolysis under glyco-oxidative stress and stabilize the F1 subcomplex.

This paper uses molecular dynamics simulations to show how rotation direction affects IF1's binding to ATP synthase, revealing atomic mechanisms for IF1 ejection and inhibition.

This study demonstrates that Enterococcus hirae V-ATPase can synthesize ATP using sodium motive force, showing reversible reactions and a Na+/ATP ratio consistent with structural symmetry.

The paper presents a cryo-EM structure of a Ca2+-bound intermediate in the Ca2+-ATPase 1 from Listeria monocytogenes, revealing insights into the inward-to-outward transition mechanism.

The paper presents a 3.2 Å resolution cryo-EM structure of a dimeric F1-like ATPase complex in Mycoplasma mobile, revealing its role in gliding motility through conserved rotary mechanisms.

The paper presents a high-throughput algorithm using AlphaFold to predict F-Type ATP synthase rotor ring stoichiometries from 8 to 27 protomers, validated with molecular dynamics simulations.

The paper measures H+ transport kinetics in mPPase using a stopped-flow assay and finds that hydrolysis, not pyrophosphate binding, directly couples to H+ transport.

The paper determines the structure of yeast RAVE bound to a partial V1 complex using cryo-EM, revealing its role in facilitating V1-VO reassociation.

This study identifies TgIF1's role in mitochondrial cristae maintenance and stress response in Toxoplasma gondii, showing its importance under hypoxia and oxidative stress.

The paper solves the cryo-EM structure of an axle-less F1-ATPase and shows that the complete γ subunit is important for efficient ATP binding.

This study investigates the N-terminal regulation of ACA8 calcium pump in A. thaliana, showing that residues 20-35 and Phe32 are critical for autoinhibition and calmodulin activation.

The paper determines the high-resolution structure of V-ATPase in native synaptic vesicles using cryo-EM, revealing interactions with cholesterol and synaptophysin.

This study investigates the reversible assembly and disassembly of V-ATPase during lysosome regeneration in continuously fed cells, showing its role in regulating endolysosomal pH.

The paper reports the first structure of the Mg2+ transporter MgtA using cryo-EM, revealing its dimeric form and mechanism of Mg2+ transport.

The paper identifies six states of Enterococcus hirae V-ATPase using cryo-EM, revealing non-uniform rotor rotation during turnover.

This study identifies a new inhibited conformation of KdpFABC, termed E1-P tight, resulting from KdpBS162 phosphorylation, which represents an off-cycle state in P-type ATPases.

The paper reports cryo-EM structures of the ATP synthase dimer from Trypanosoma brucei, identifying an ancestral subunit-g/e module critical for oligomerization.