Biochemical and Molecular Research

The paper investigates how cytoophidium complexes formed by different metabolic enzymes relate to cell fates in yeast, showing that blocking CTPS cytoophidium assembly disrupts quiescence and affects NAD biosynthesis.

This paper shows that mycobacterial MutT1, through its Nudix hydrolase domain, functions as a protein phosphatase to regulate NDK-Pi levels and prevent the conversion of 8-oxo-dGDP to 8-oxo-dGTP, thereby reducing A to C mutations.

The paper investigates how free amino acids affect the EDTA-induced inactivation of rat liver NPP/PDE Enpp3 and identifies Enpp3 as a 2,3-cGAMP hydrolase.

This paper shows that DHODH inhibitors enhance the antiviral effect of 4'-FlU against multiple RNA viruses, including influenza, SARS-CoV-2, and Ebola virus.

This paper characterizes Giardia intestinalis deoxyadenosine kinase, showing it forms a tetramer with high substrate affinity and sensitivity to deoxyadenosine analogues, making it a potential drug target.

This study shows that inhibiting purine biosynthesis with mizoribine prevents weight gain and improves metabolic health in mice on a high-fat diet by increasing thermogenesis and reducing food intake.

This study identifies pathogenic CAD mutations that disrupt oligomerization, showing their impact on enzymatic activity and nucleotide synthesis.

This study compares cytoophidium assembly in Saccharomyces cerevisiae and Schizosaccharomyces pombe under varying environmental and intracellular conditions, finding differential responses to temperature, pH, and CTPS overexpression.

This study identifies SLC29A homologs in C. elegans that mediate nucleoside coordination between soma and germline, revealing guanosine as critical for reproduction.

This paper reveals the activation pathway of bemnifosbuvir to its active form AT-9010, showing the required enzymes and structural insights into the process.

This paper shows that expressing ScURA in mammalian cells allows pyrimidine biosynthesis independent of mitochondrial electron transport, enabling growth in uridine-deficient medium and improving mitochondrial disease models.

The paper identifies a mechanism where dTMP equilibration via gap junctions enables collective tumor growth in cells lacking canonical dTMP synthesis.

This study shows that IMPDH2 filaments protect neurons from degeneration in AMPD2 deficiency by preventing GTP deprivation.

The paper shows that IMPDH2 is required for tail regeneration in Xenopus tropicalis and forms filaments in regenerating tissue under high GTP demand.

This study presents crystal structures of Streptococcus pneumoniae PYK bound to nucleotides, revealing structural basis for GTP selectivity and its role in nucleotide homeostasis.

This study identifies WDR62's role in regulating purine metabolism via the HSP70/HSP90 chaperone network, linking its dysfunction to microcephaly through metabolic enzyme instability.

The paper identifies URA6 mutations that confer 5-FOA resistance in yeast, showing cross-resistance to other fluorinated compounds and revealing a reduction in uridylate kinase activity.

The paper studies dynamic cytoophidia in Drosophila late oogenesis using live cell imaging, revealing heterogeneity and dynamic patterns in nurse cells and oocytes.

The paper quantifies the substrate promiscuity of Escherichia coli xanthine oxidase by showing it can use guanine as a substrate, expanding its known metabolic role.

This study shows that purine biosynthesis pathways are essential for myogenesis in Xenopus laevis by affecting Myogenic Regulatory Factors expression.

This study investigates the spatiotemporal regulation of de novo and salvage purine synthesis during brain development and finds that their utilization shifts from de novo in embryonic stages to salvage in postnatal-adult stages, with implications for mTOR signaling and brain morphology.

The paper shows that gap junctions enable nucleotide sharing between cells, buffering replication stress and affecting cell sensitivity to dNTP depletion.

This study investigates purine recycling deficiency in Drosophila, showing metabolic and neurobehavioral disturbances similar to Lesch-Nyhan disease in humans.

This study investigates how one-carbon metabolic enzymes regulate cell cycle progression and metabolome in Saccharomyces cerevisiae, showing distinct effects of enzyme loss on cell size, growth, and metabolic profiles.

This study investigates the role of cytoophidia in Drosophila follicle epithelium integrity using a mutation that disrupts cytoophidium formation.

The paper shows that CTP synthase filaments (cytoophidia) are required to maintain binucleation in Drosophila male accessory gland cells.

The paper shows that CTP synthase does not form cytoophidia in Drosophila interfollicular stalk cells, and links this to low Myc levels and resistance to DON treatment.

This study investigates the effects of ADSL deficiency on neurodevelopment, showing both SAICAr-dependent and independent mechanisms contributing to pathologies like microcephaly and autism.

The paper uses control theory and buffer analysis to show that buffering enables independent control of ATP and adenylate energy ratios in metabolism, supported by human and mouse data.

This study identifies MoAde4 as essential for purine biosynthesis in Magnaporthe oryzae, showing its role in conidiation and host colonization, not appressorium formation.