Biochemical and Molecular Research

This study investigates the structural and functional organization of the de novo purine nucleotide biosynthetic pathway enzymes in Pseudomonas aeruginosa, revealing a metabolon-like complex with potential antibiotic implications.

This study identifies PRPS-PRPSAP complexes as regulators of histone chaperone interactions, linking nucleotide biosynthesis to chromatin assembly.

This study shows NUDT5 regulates the effectiveness of nucleoside analog drugs by coordinating purine synthesis and PRPP allocation through its interaction with PPAT.

This study shows that reduced hepatic pyrimidine synthesis during fasting and obesity promotes insulin resistance and obesity by increasing hepatic glucose production and decreasing insulin clearance.

This study identifies NUDT5 as a regulator of purine metabolism and thiopurine sensitivity by interacting with PPAT, independent of its known enzymatic activity.

This study investigates how the alarmones ppGpp and pppGpp differentially regulate the nucleosidase PpnN in E. coli, revealing its role in bacterial survival under antibiotic stress and uncovering a conserved catalytic mechanism shared with plant cytokinin signaling proteins.

This study shows that succinate dehydrogenase supports de novo purine synthesis in cancer cells, and its inhibition reduces proliferation by impairing purine biosynthesis and inducing a compensatory salvage pathway.

The paper identifies purine nucleotides as competitive inhibitors of apo-GOT1 by showing they bind to the PLP pocket and destabilize the enzyme in its cofactor-free state.

This study examines the effects of slc43a3 knockout on metabolic and physical activity in mice, finding sex-specific differences in oxygen consumption, circadian rhythms, and blood pressure.

This study identifies purine nucleobases as immunometabolites that enhance CD8+ T cell effector function by enabling a metabolic switch from de novo synthesis to salvage pathway.

This study investigates how cancer cells adapt to purine shortage stress and identifies vulnerabilities in purine metabolism and microtubules as therapeutic targets.

This study identifies that combining DHODH inhibition with BCL-XL targeting synergistically induces apoptosis in pancreatic cancer, offering a new combination therapy strategy.

This study traces the evolutionary origins of mammalian PRPS genes and shows how their duplication and specialization enable functional multimeric complexes with regulatory features.

This study identifies picornavirus proteins that inhibit de novo nucleotide synthesis during infection using metabolomics and recombinant virus analysis.

This study shows that Mycobacterium tuberculosis CTP synthase forms unique filaments that stabilize enzyme activity and resist inhibition, with mutations affecting filament formation under positive selection in clinical isolates.

The paper determines the structure of HPAMPD, a mollusk adenosine deaminase with nucleotide specificity, and shows how it binds the 5-monophosphate group of AMP through specific structural elements.

This paper shows that an IMPDH2 variant linked to neurodevelopmental disorders disrupts purine biosynthesis and somitogenesis in Xenopus tropicalis by affecting filament assembly and GTP sensitivity.

The paper identifies a non-enzymatic role of NUDT5 in repressing purine de novo synthesis by scaffolding PPAT, with implications for cancer treatment and MTHFD1 deficiency.

The paper identifies genes and metabolic regulators of nucleotide synthesis using uridine salvage and CRISPR-Cas9 screening, revealing NUDT5's role in maintaining nucleotide balance and its impact on chemotherapy resistance.

This paper identifies m-APTA, an MTA analogue that reduces NBA toxicity without hypothermia, improving safety for MTAP-deficient cancer treatment.

The paper investigates how the monomer/dimer switch in plant adenosine kinase (ADK) modulates enzyme activity and establishes a negative feedback mechanism to regulate adenosine and AMP levels.

The paper identifies Complex II's role in de novo purine biosynthesis in AML using CRISPR and metabolomic data, showing it as a therapeutic target.

The paper identifies pyruvate kinase's role in GTP synthesis during glycolysis in bacteria, showing it reduces guanosine toxicity and contributes to NTP production.

This study identifies CTP as a regulator of hCTPS1 filamentation, revealing its structural basis and evolutionary conservation.

This study examines IMPDH cytoophidia in pancreatic islets under normal and diabetic conditions, showing morphological changes linked to impaired glucose tolerance.

The paper shows that deleting both NT5C1A and NT5C2 in mice lowers blood sugar by improving muscle insulin response and reducing liver glucose production.

This study characterizes two PPK2 enzymes, showing their broad substrate specificity and ability to selectively synthesize pyrimidine nucleoside diphosphates with high yield.

The paper investigates how cytoophidium complexes formed by different metabolic enzymes relate to cell fates in yeast, showing that blocking CTPS cytoophidium assembly disrupts quiescence and affects NAD biosynthesis.

This paper shows that mycobacterial MutT1, through its Nudix hydrolase domain, functions as a protein phosphatase to regulate NDK-Pi levels and prevent the conversion of 8-oxo-dGDP to 8-oxo-dGTP, thereby reducing A to C mutations.