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This paper demonstrates that ATR-FTIR spectroscopy with regression models can rapidly and non-destructively quantify DNA methylation and hydroxymethylation in DNA samples, including circulating tumor DNA.

This study synthesizes and evaluates triazole-pyrazine derivatives for anti-HSV-1 and antioxidant activity, finding compounds 5f and 5t show improved antiviral efficacy compared to Acyclovir.

A reconstituted, direct-RNA-sequencing transcription assay on 1,000 human promoter-proximal sequences identified a TFIIS-insensitive consensus super pause, whose cryo-EM structure shows a one-nucleotide sidetracked polymerase state.

This study evaluates how detergent selection and timing affect thermal stability proteomics results, finding that DDM improves detection of some drug-target interactions but may obscure others.

The paper uses molecular dynamics simulations to study the dynamic interactions between Tau protein and tubulin C-terminal tails, revealing sequence-dependent effects and disorder-disorder interplay.

The paper develops and validates a genetically encoded fluorescent sensor, iG6PSnFR, for measuring glucose-6-phosphate dynamics across multiple biological contexts.

This study identifies MSTO1 as a TRiC assembly factor, showing that its loss leads to reduced TRiC levels and mitochondrial dysfunction, linking cytosolic proteostasis to mitochondrial health.

This study analyzes NADH conformation and interaction patterns across 345 protein structures to uncover general principles of cofactor recognition and interaction.

The paper presents high-resolution cryo-EM structures of LHCII at different pH levels, revealing minimal conformational changes and insights into lutein conformations and energy quenching mechanisms.

This study uses molecular modeling and simulations to clarify how IRE1 detects unfolded proteins, revealing binding pockets and interactions that stabilize oligomers and prevent larger complex formation.

This study characterizes the conformational states of human plasminogen using structural biology techniques, revealing closed and open conformations in recombinant and plasma-derived variants.

This paper introduces EYClamp, a de novo designed enzyme for photo-proximity labeling of E3 ligase neighborhoods in live cells, identifying over 1,500 candidate neighbors for four E3 ligases.

The paper shows that extracellular vesicles from HGSNAT-overexpressing cells can rescue MPS IIIC neuron defects by delivering the enzyme to lysosomes.

This study uses anaerobic time-resolved serial crystallography to capture CO dissociation and rebinding in [FeFe]-hydrogenase, revealing a rigid catalytic architecture.

This study identifies how cFP binds ToxRS in V. vulnificus to activate virulence genes by stabilizing the ToxR-ToxS heterodimer and enhancing promoter binding.

The paper compares the skeletal proteomes of three coral species to identify a conserved biomineralization toolkit across different environments and symbiotic statuses.

The paper determines the structure of an immature proPC1/3 variant and identifies a conformational checkpoint for ER export, explaining the functional impact of PCSK1 mutations.

This study investigates how Munc18 influences the order and pathway of SNARE complex assembly, revealing a concerted mechanism that promotes functional fusion.

This study reprograms protein interfaces between adenylation and carrier protein domains in NRPSs to enable non-cognate pairings and enhance natural product production.

This paper reports crystal structures of a far-red CBCR in three light-absorbing states, revealing new insights into spectral tuning and light signaling mechanisms through protein-chromophore interactions.