A. Darszon

Fluid flow reconstruction around a free-swimming sperm in 3D

This study reconstructs fluid flow around a free-swimming human sperm using 3D microscopy and numerical models, revealing counter-rotating vortices in the flow pattern.

The sodium-proton exchangers sNHE and NHE1 control plasma membrane hyperpolarization in mouse sperm.

This study identifies Na+/H+ exchangers as key regulators of plasma membrane hyperpolarization in mouse sperm, challenging the role of PKA and revealing a novel pathway involving cAMP, calcium, and SLO3 K+ channels.

Reorganization of the Flagellum Scaffolding Induces a Sperm Standstill During Fertilization

This study shows that reorganization of the flagellum's F-actin network during sperm-egg fusion leads to midpiece contraction and motility cessation.

Feature-Based 3D+t Descriptors of Hyperactivated Human Sperm Beat Patterns

This paper develops 3D+t descriptors to classify hyperactivated human sperm motility patterns using feature-based vectors, showing they can distinguish hyperactivated from nonhyperactivated sperm without prior training.

The human sperm head spins with a conserved direction during swimming in 3D

This study directly measures human sperm head spinning in 3D using 4D microscopy, revealing a consistent counterclockwise direction regardless of experimental conditions.

Cdc42 activity is essential for the interplay between cAMP/PKA pathway and CatSper function

The paper shows that Cdc42 activity is crucial for the interaction between the cAMP/PKA pathway and CatSper function in sperm, affecting Ca2+ transport and fertilization.

Computer-assisted segmentation-free analysis for long-term assessment of flagellar beating in free swimming human spermatozoa: the role of emptying calcium stores

This paper introduces a segmentation-free method to analyze flagellar beating in human spermatozoa over long periods, revealing nonlinear effects of intracellular Ca2+ on beat frequency and amplitude.

Sperm chemotaxis is driven by the slope of the chemoattractant concentration field

This study shows that sea urchin sperm chemotaxis depends on the slope of chemoattractant concentration gradients, mediated by coupling two oscillators: the stimulus function and intracellular calcium changes.

The human sperm beats anisotropically and asymmetrically in 3D

This paper uses 3D imaging and mathematical analysis to show human sperm flagella beat anisotropically and asymmetrically, revealing a novel mechanism that creates the illusion of symmetry in 2D observations.

CaV3.1 channel pore pseudo-symmetry revealed by selectivity filter mutations in their domains I/II

This study examines how mutations in domains I and II of CaV3.1 channels affect gating properties, revealing differential effects that suggest pore pseudo-symmetry.

Non-enzymatically oxidized arachidonic acid regulates mouse spermatogenic cell T-type Ca2+ currents

This study shows that non-enzymatically oxidized arachidonic acid inhibits T-type Ca2+ currents in mouse spermatogenic cells, potentially contributing to infertility and apoptosis.