Author

A. Pruss

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Immunology· 2 Bioengineering· 1

Signature Assays, Organisms & Methods

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Research Focus:drug response / drug discovery· 3 Approach:genetic perturbation / CRISPR· 3 Organism:human· 3 Research Focus:cell type annotation· 1 Assay:CRISPR screen· 1 Assay:proteomics· 1

Frequent Co-Authors

D. L. Wagner· 3 shared H.-D. Volk· 3 shared J. Kath· 3 shared M. Schmueck-Henneresse· 3 shared M. Stein· 3 shared P. Reinke· 3 shared W. Du· 3 shared A. Kuenkele· 2 shared

Activity by Year

2021-2023 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 45 mutual co-author links

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3 shared papersImmunology

D. L. Wagner

Mutual co-authors in cluster

Author profile →

3 papers

Recent·Most cited

Integration of ζ-deficient CARs into the CD3-zeta gene conveys potent cytotoxicity in T and NK cells

J. Kath, C. Franke, V. Drosdek, W. Du, V. Glaser, C. Fuster-Garcia, M. Stein, T. Zittel, S. Schulenberg, C. E. Porter, L. Andersch, A. Kuenkele, J. Alcaniz, J. Hoffmann, H. Abken, , D. L. Wagner

This paper develops a method to integrate ζ-deficient CARs into the CD3ζ gene to create functional CAR-T and CAR-NK cells with improved safety and efficacy for off-the-shelf therapies.

bioRxiv · Nov 10, 2023 · PreprintImmunology

Combining different CRISPR nucleases for simultaneous knock-in and base editing prevents translocations in multiplex-edited CAR T cells

V. Glaser, C. L. Flugel, J. Kath, W. Du, V. Drosdek, C. Franke, M. Stein, A. Pruss, M. Schmueck-Henneresse, H.-D. Volk, P. Reinke, D. L. Wagner

This paper combines CRISPR nucleases to simultaneously knock-in and base edit CAR T cells, reducing translocations to 1.5% and improving safety.

bioRxiv · Nov 11, 2022Bioengineering

Fast, efficient and virus-free generation of TRAC-replaced CAR T cells

J. Kath, W. Du, B. Thommandru, R. Turk, L. Amini, M. Stein, T. Zittel, S. Martini, L. Ostendorf, A. Wilhelm, L. Akyuez, A. Rehm, U. E. Hoepken, A. Pruss, A. Kuenkele, , D. L. Wagner

This paper presents a virus-free method to generate CAR T cells by inserting CARs into the TRAC locus using CRISPR-Cas and HDR, achieving up to 68% TCR-to-CAR replacement.

bioRxiv · Feb 14, 2021Immunology