A. S. Gladfelter

This study identifies dynamical control as a mechanism enabling the formation of demixed biomolecular condensates by showing that asynchronous molecular production is required for their coexistence.

This study shows septin curvature sensing is kinetically determined, influenced by bulk concentration and available membrane curvatures, through cooperative assembly of septin oligomers.

The paper develops a method for smFISH in Neurospora crassa by introducing a chitinase for RNase-free cell wall digestion and providing image analysis tools.

The paper shows membrane surfaces control the size and position of ribonucleoprotein condensates by altering diffusion rates of proteins and nucleic acids.

This study shows that a specific sequence motif in the Whi3 protein promotes dilute phase oligomerization, which opposes phase separation and alters the composition and material properties of ribonucleoprotein condensates.

This study shows septin filaments are isodesmic polymers, and regulatory proteins modulate their biophysical properties to form different structures in yeast.

This study investigates the role of amphipathic helices in septin proteins, showing their involvement in membrane curvature sensing and filament bundling.

The paper develops a 3D neural network-based pipeline to track cytosolic nanoparticles in fungi, revealing spatial heterogeneity in diffusivity within and between cells.

This study shows that alternative splicing of FXR1 regulates its function in muscle development by controlling RNA-dependent condensates and disease-related mis-splicing.

This study identifies an amphipathic helix in septins that enables curvature sensing at sub-micrometer scales, independent of filament length.

The paper introduces LITE microscopy, a technique that improves resolution and brightness while reducing photodamage in fluorescence imaging.