K. M. Ruff

Tunable metastability of condensates reconciles their dual roles in amyloid fibril formation

The paper shows that separable interactions in A1-LCD allow tunable metastability of condensates, which can suppress amyloid fibril formation even with ALS mutations.

SOURSOP: A Python package for the analysis of simulations of intrinsically disordered proteins

SOURSOP is a Python package for analyzing simulated conformational ensembles of intrinsically disordered proteins, offering tools to quantify sequence-specific properties.

Dynamical control enables the formation of demixed biomolecular condensates

This study identifies dynamical control as a mechanism enabling the formation of demixed biomolecular condensates by showing that asynchronous molecular production is required for their coexistence.

Uncovering molecular grammars of intrinsically disordered regions that organize nucleolar fibrillar centers

The paper identifies molecular grammars in intrinsically disordered regions of nucleolar proteins that drive phase separation to form nucleolar substructures.

Connecting sequence features within the disordered C-terminal linker of B. subtilis FtsZ to function and bacterial cell division

This paper investigates how sequence patterns in the disordered C-terminal linker (CTL) of Bacillus subtilis FtsZ influence autoregulatory interactions and bacterial cell division.

Competing interactions give rise to two-state behavior and switch-like transitions in charge-rich intrinsically disordered proteins

This paper uses atomistic simulations to show polyampholytic IDPs exhibit two-state behavior between globules and self-avoiding walks, with sequence-specific temperature-dependent preferences.

Sequence grammar underlying unfolding and phase separation of globular proteins

The paper investigates how sequence grammars of unfolded globular proteins drive phase separation and formation of UPODs, highlighting the role of hydrophobic residues and their impact on disease-related interactions.

Mechanistic inferences from analysis of measurements of protein phase transitions in live cells

This paper develops a supervised method to classify phase transitions in live cells using DAmFRET data, revealing sequence features that drive prion-like behavior and enabling quantitative analysis of nucleation probabilities.

Ligand Effects on Phase Separation of Multivalent Macromolecules

This study uses theory and computation to show how ligands regulate scaffold phase behavior by binding to specific sites, affecting condensate stability and structure.

Proteomic and functional mapping of cardiac NaV1.5 channel phosphorylation reveals multisite regulation of surface expression and gating

The paper identifies 42 phosphorylation sites on NaV1.5 channels in mouse hearts and shows their roles in regulating channel expression and gating.

Molecular basis for dyneinopathies reveals insight into dynein regulation and dysfunction

This study identifies molecular mechanisms of dyneinopathies using yeast to analyze disease-linked mutants and shows a correlation between dynein dysfunction severity and disease type.

Tadpole-like conformations of huntingtin exon 1 with expanded polyglutamine engenders novel interactions in cells

The paper investigates the structure of Httex1 with expanded polyQ and finds that its tadpole-like conformation increases interactions in cells, potentially contributing to neurotoxicity.